Overcoming Cabbage Crossing Incompatibility by the Development and Application of Self-Compatibility-QTL- Specific Markers and Genome-Wide Background Analysis.

Cabbage hybrids, which clearly present heterosis vigor, are widely used in agricultural production. We compared two haplotype (Class II) cabbage inbred-lines 87-534 and 94-182: the former is highly SC while the latter is highly SI; sequence analysis of SI-related genes including , , , , and indicates the some SNPs in and of 87-534; semi-quantitative analysis indicated that the SI-related genes were transcribed normally from DNA to mRNA. To unravel the genetic basis of SC, we performed whole-genome mapping of the quantitative trait loci (QTLs) governing self-compatibility using an F population derived from 87-534 × 96-100. Eight QTLs were detected, and high contribution rates (CRs) were observed for three QTLs: (54.8%), (14.1%) and (11.2%). 06-88 (CB201 × 96-100) yielded an excellent hybrid. However, F seeds cannot be produced at the anthesis stage because the parents share the same haplotype (, class I). To overcome crossing incompatibility, we performed rapid introgression of the self-compatibility trait from 87-534 to 96-100 using two self-compatibility-QTL-specific markers, BoID0709 and BoID0992, as well as 36 genome-wide markers that were evenly distributed along nine chromosomes for background analysis in recurrent back-crossing (BC). The transfer process showed that the proportion of recurrent parent genome (PRPG) in BCF was greater than 94%, and the ratio of individual SC plants in BCF reached 100%. The newly created line, which was designated SC96-100 and exhibited both agronomic traits that were similar to those of 96-100 and a compatibility index (CI) greater than 5.0, was successfully used in the production of the commercial hybrid 06-88. The study herein provides new insight into the genetic basis of self-compatibility in cabbage and facilitates cabbage breeding using SC lines in the male-sterile (MS) system.