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小麦（L.）根系中脱落酸依赖和非依赖途径下渗透响应基因的转录组分析

Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat ( L.) roots.

作者信息

Li Chunxi, Zhang Wenli, Yuan Meng, Jiang Lina, Sun Bo, Zhang Daijing, Shao Yun, Liu Anqi, Liu Xueqing, Ma Jianhui

机构信息

College of Life Science, Henan Normal University, Xinxiang, The People's Republic of China.

出版信息

PeerJ. 2019 Mar 5;7:e6519. doi: 10.7717/peerj.6519. eCollection 2019.

DOI:10.7717/peerj.6519
PMID:30863676
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6407504/
Abstract

Bread wheat is one of the most important crops in the world. However, osmotic stress significantly inhibits wheat growth and development, and reduces crop yield and quality. Plants respond to osmotic stress mainly through abscisic acid (ABA)-dependent and -independent pathways. In this study, root transcriptome profiles of wheat seedlings exposed to osmotic stress and exogenous ABA were analysed to identify osmotic-responsive genes belonging to the ABA-dependent or -independent pathways. We found that osmotic stress promoted proline biosynthesis in the ABA-dependent pathway, and trehalose biosynthesis is likely promoted among soluble sugars to maintain protein bioactivity under osmotic stress. In wheat roots subjected to osmotic stress, calcium ions, and glutathione exert their functions mainly through calcium-binding protein (CaM/CML) and glutathione--transferase, respectively, depending on both pathways. In addition, a complex relationship among phytohormones signal transduction was observed in response to osmotic stress. The findings of this study deepen our understanding of the molecular mechanisms of osmotic-stress resistance, and provide several candidate osmotic-responsive genes for further study.

摘要

面包小麦是世界上最重要的作物之一。然而，渗透胁迫显著抑制小麦的生长发育，并降低作物产量和品质。植物主要通过脱落酸（ABA）依赖和非依赖途径对渗透胁迫作出反应。在本研究中，分析了暴露于渗透胁迫和外源ABA下的小麦幼苗根系转录组图谱，以鉴定属于ABA依赖或非依赖途径的渗透响应基因。我们发现，渗透胁迫在ABA依赖途径中促进脯氨酸生物合成，在渗透胁迫下，海藻糖生物合成可能在可溶性糖中得到促进，以维持蛋白质生物活性。在遭受渗透胁迫的小麦根系中，钙离子和谷胱甘肽分别主要通过钙结合蛋白（CaM/CML）和谷胱甘肽转移酶发挥作用，这取决于这两种途径。此外，在对渗透胁迫的响应中，观察到植物激素信号转导之间存在复杂的关系。本研究结果加深了我们对渗透胁迫抗性分子机制的理解，并为进一步研究提供了几个候选渗透响应基因。

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