Guo Lei, Wang Le, Li Xiangrong, Xu Xiaowen, Guo Jiacai, Wang Xintong, Yang Weiqin, Xu Fuxuan, Li Fuhou
Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005 China.
3 Biotech. 2020 Feb;10(2):54. doi: 10.1007/s13205-020-2067-z. Epub 2020 Jan 21.
Questin has favorable applications. Fractional factorial design, Box-Behnken design, and response surface methodology were adopted to optimize the fermentation conditions of the marine-derived fungus, HN4-13, thereby enhancing questin production. Optimal fermentation conditions in a 500-mL conical flask with 200 mL of medium were 4% soluble starch, 0.9% beef extract, 4% NaCl, 0.05% NaHPO, pH 6, 2% inoculum size, and shaking at 28 ℃ and 160 rpm/min for 7 days. The production of questin can achieve 64.93 ± 4.55 mg/L, with no significant difference from the predicted value (66.27 mg/L). Thus, this optimized process of questin production is feasible. Such production is 17-fold higher than that of the basal Sabouraud's dextrose medium. Results indicate the potential of . HN4-13 in the large-scale production of questin through fermentation.
喹啉有良好的应用。采用分数析因设计、Box-Behnken设计和响应面方法对海洋来源真菌HN4-13的发酵条件进行优化，从而提高喹啉产量。在装有200 mL培养基的500 mL锥形瓶中的最佳发酵条件为：4%可溶性淀粉、0.9%牛肉浸出物、4%氯化钠、0.05%磷酸氢二钠、pH 6、2%接种量，于28℃、160 rpm/min振荡培养7天。喹啉产量可达64.93±4.55 mg/L，与预测值（66.27 mg/L）无显著差异。因此，这种优化的喹啉生产工艺是可行的。这种产量比基础沙氏葡萄糖培养基高17倍。结果表明HN4-13在通过发酵大规模生产喹啉方面具有潜力。
