Rasmussen L, Merigan T C
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3957-61. doi: 10.1073/pnas.75.8.3957.
Lymphocyte blast transformation and interferon production in mononuclear cell culture prepared on Ficoll-Hypaque gradients from individuals with herpes simplex virus-I infection were enhanced by a disease recurrence. Responses to both herpes simplex virus-2 and phytohemagglutinin were unaltered. Transformation to herpes simplex virus-I antigen was adversely affected by depleting either thymus-derived (T) lymphocytes or bone marrow-derived (B) lymphocytes together with monocytes from cultures. The transformation response was reconstructed when the selectively depleted lymphocyte populations were recombined. X-irradiation of either T or B lymphocytes and monocytes showed that T lymphocytes incorporated [3H]thymidine with the aid of a radioresistant non-rosetting cell, probably a monocyte. Depletion of B lymphocytes and monocytes, but not of T lymphocytes, resulted in reduction in interferon production. Irradiated B lymphocytes and monocytes failed to produce significant quantities of interferon, suggesting that a radiosensitive B cell was a major interferon source.
来自单纯疱疹病毒I型感染个体、通过Ficoll-泛影葡胺梯度制备的单核细胞培养物中的淋巴细胞转化和干扰素产生，会因疾病复发而增强。对单纯疱疹病毒2型和植物血凝素的反应未改变。从培养物中耗尽胸腺来源的（T）淋巴细胞或骨髓来源的（B）淋巴细胞以及单核细胞会对向单纯疱疹病毒I型抗原的转化产生不利影响。当选择性耗尽的淋巴细胞群体重新组合时，转化反应得以重建。对T或B淋巴细胞以及单核细胞进行X射线照射表明，T淋巴细胞借助一种抗辐射的非凝集细胞（可能是单核细胞）掺入[3H]胸腺嘧啶核苷。耗尽B淋巴细胞和单核细胞而非T淋巴细胞会导致干扰素产生减少。经照射的B淋巴细胞和单核细胞未能产生大量干扰素，这表明放射敏感的B细胞是干扰素产生的来源。
