Shillitoe E J, Wilton J M, Lehner T
Scand J Immunol. 1978;7(5):357-66. doi: 10.1111/j.1365-3083.1978.tb00465.x.
Lymphocytes of seven patients with primary herpetic infection, twenty-three patients with recurrent herpes labialis and of nineteen control subjects were separated into T and B enriched cells by the use of nylon wool columns. In the absence of a herpetic infection the thymidine incorporation and macrophage migration inhibition responses to herpes simplex virus (HSV), Candida albicans and PPD, and the thymidine incorporation induced by PHA were functions of T cells. When a herpetic infection was present, the unfractionated lymphocyte response to HSV was increased, as measured by thymidine incorporation, but the T cell response was unchanged. However, T cells did show an increased response to HSV when prepared by elimination of cells forming rosettes with zymosan-complement. T cells of some patients were stimulated by contact with zymosan, and this correlated with the response to C. albicans. It is suggested that lymphocyte responses to HSV in man are mediated by T cells, but that these cells are specifically retained by nylon wool columns at the time of a herpetic infection. This may be associated with acquisition of an Fc receptor by the sensitized T cells.
利用尼龙毛柱将7例原发性疱疹感染患者、23例复发性唇疱疹患者以及19名对照者的淋巴细胞分离为富含T细胞和B细胞的细胞群。在无疱疹感染的情况下，对单纯疱疹病毒（HSV）、白色念珠菌和结核菌素纯蛋白衍生物（PPD）的胸腺嘧啶核苷掺入及巨噬细胞游走抑制反应，以及由PHA诱导的胸腺嘧啶核苷掺入均为T细胞的功能。当存在疱疹感染时，通过胸腺嘧啶核苷掺入测定，未分级淋巴细胞对HSV的反应增强，但T细胞反应未改变。然而，当通过去除与酵母聚糖 - 补体形成花环的细胞来制备T细胞时，T细胞对HSV的反应确实增强。一些患者的T细胞通过与酵母聚糖接触而受到刺激，这与对白色念珠菌的反应相关。提示人淋巴细胞对HSV的反应由T细胞介导，但在疱疹感染时这些细胞被尼龙毛柱特异性保留。这可能与致敏T细胞获得Fc受体有关。
