Department of Biology, Saint Louis University, Saint Louis, Missouri.
Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, Saint Louis, Missouri.
J Biomed Mater Res B Appl Biomater. 2019 Nov;107(8):2620-2628. doi: 10.1002/jbm.b.34351. Epub 2019 Mar 13.
Manuka honey is an ancient remedy to improve wound healing; however, an effective delivery system is needed to facilitate extended release of honey into wounds. We developed an electrospun dermal regeneration template consisting of a poly (ε-caprolactone) (PCL) scaffold embedded with 1%, 5%, 10%, or 20% manuka honey. In vitro studies demonstrated that honey PCL scaffolds were not toxic to macrophages, and they allowed for macrophage infiltration into the scaffolds. Vascular endothelial growth factor (VEGF), a marker of angiogenesis, was released by macrophages cultured with scaffolds and macrophage/scaffold conditioned media promoted endothelial cell tube formation in an angiogenesis assay. In a full thickness murine wound model, the scaffolds prevented rapid wound contraction. In vivo, cells infiltrated the scaffolds by post-wounding day 7, but the honey scaffolds did not affect collagen deposition at that time. In summary, preliminary studies investigating the effect of honey on tissue repair show that scaffolds prevent rapid wound contraction, allow for cell infiltration, and promote angiogenesis. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2620-2628, 2019.
麦卢卡蜂蜜是一种古老的疗法，可以促进伤口愈合；然而，需要一种有效的给药系统来促进蜂蜜向伤口的持续释放。我们开发了一种电纺真皮再生模板，由聚己内酯（PCL）支架组成，其中嵌入了 1%、5%、10%或 20%的麦卢卡蜂蜜。体外研究表明，蜂蜜 PCL 支架对巨噬细胞无毒，并且允许巨噬细胞渗透到支架中。血管内皮生长因子（VEGF）是血管生成的标志物，在支架上培养的巨噬细胞中释放出来，巨噬细胞/支架条件培养基在血管生成测定中促进内皮细胞管形成。在全层小鼠伤口模型中，支架防止了伤口的快速收缩。在体内，细胞在创伤后第 7 天渗透到支架中，但在那个时候，蜂蜜支架并没有影响胶原蛋白的沉积。总之，初步研究表明，蜂蜜对组织修复的影响表明，支架可以防止伤口快速收缩，允许细胞渗透，并促进血管生成。2019 年 Wiley 期刊公司。J 生物医学材料研究部分 B:应用生物材料 107B:2620-2628。
