Wei Chenxuan, Gong Ting, Pow Edmond H N, Botelho Michael G
Department of Prosthodontics, Faculty of Dentistry, the University of Hong Kong, Hong Kong, China.
J Investig Clin Dent. 2019 Aug;10(3):e12407. doi: 10.1111/jicd.12407. Epub 2019 Mar 13.
The aim of the present study was to investigate the initial stem cell and pre-osteoblast cell adhesion and oxidative response on zirconia in comparison with titanium.
Human dental pulp stem cells (DPSC) and murine pre-osteoblasts (MC3T3-E1) cells were cultured on zirconia and titanium surfaces, and at 3-, 12-, and 24-hour intervals, cell viability and morphology were determined with tetrazolium based colorimetric assay, scanning electron microscopy, and immunofluorescence analysis. The in situ reactive oxygen species level of both cells on each material surface was examined after 24-hour culture.
Both DPSC and MC3T3-E1 cells revealed comparable morphological features during 24-hour cell adhesion processes, with cells continued expanding of cell size and increasing of cell viability on titanium and zirconia surfaces during 24-hour culture. Zirconia demonstrated relatively higher mean cell viability compared to titanium within 24-hour culture, with significantly higher DPSC viability at 12 hours after seeding (P < 0.05). Relatively higher mean reactive oxygen species levels in both DPSC and MC3T3E1 were found on zirconia surfaces after 24-hour culture compared to titanium.
From the results, zirconia as a potential dental implant substrate demonstrated equivalent or better initial cellular responses compared to titanium.
本研究旨在比较氧化锆与钛对干细胞和前成骨细胞的初始黏附及氧化反应。
将人牙髓干细胞（DPSC）和小鼠前成骨细胞（MC3T3-E1）培养于氧化锆和钛表面，每隔3小时、12小时和24小时，采用基于四氮唑的比色法、扫描电子显微镜和免疫荧光分析来测定细胞活力和形态。培养24小时后，检测每种材料表面上两种细胞的原位活性氧水平。
在24小时的细胞黏附过程中，DPSC和MC3T3-E1细胞均呈现出相似的形态特征，在24小时培养期间，钛和氧化锆表面上的细胞大小持续扩大，细胞活力增加。在24小时培养内，氧化锆的平均细胞活力相对高于钛，接种后12小时DPSC活力显著更高（P < 0.05）。与钛相比，培养24小时后，氧化锆表面上DPSC和MC3T3E1的平均活性氧水平相对更高。
结果表明，氧化锆作为一种潜在的牙种植体基质，与钛相比，表现出同等或更好的初始细胞反应。
