Daniel P T, Falcioni F, Berg A U, Berg P A
Methods Find Exp Clin Pharmacol. 1986 Mar;8(3):139-45.
The influence of Cianidanol (Ci), a cytoprotective radical scavenger, on peripheral blood mononuclear cells (PBMC) was assessed with respect to its immunomodulatory function. In previous studies performed in our laboratory, a bidirectional influence of Ci on the immune response was observed, depending on its concentration. In order to elucidate this effect, the influence of Ci on macrophage (M phi) and B-cell function was investigated. A marked dose-dependent suppression of M phi phagocytosis by Ci could be detected. Furthermore, PGE2 synthesis of non-activated and PHA-activated PBMC was inhibited in the presence of Ci. This effect was shown to be due to an inhibition of M phi cyclooxygenase. It was also demonstrated that neither spontaneous nor Staphylococcus aureus Cowan I induced proliferation of highly purified B-cells was enhanced by Ci. Similar results were obtained by measuring the influence of Ci on immunoglobulin secretion of purified B-cells, exposed to Klebsiella membrane preparations. From these data it can be concluded that the previously described enhancing effect of Ci on immunoglobulin secretion is probably T-cell mediated.
对细胞保护自由基清除剂西尼达醇（Ci）在外周血单个核细胞（PBMC）免疫调节功能方面的影响进行了评估。在我们实验室之前进行的研究中，观察到Ci对免疫反应具有双向影响，这取决于其浓度。为了阐明这种作用，研究了Ci对巨噬细胞（M phi）和B细胞功能的影响。可以检测到Ci对M phi吞噬作用有明显的剂量依赖性抑制。此外，在Ci存在的情况下，未激活的和PHA激活的PBMC的PGE2合成受到抑制。这种作用被证明是由于对M phi环氧化酶的抑制。还证明，Ci既没有增强高度纯化的B细胞的自发增殖，也没有增强金黄色葡萄球菌Cowan I诱导的增殖。通过测量Ci对暴露于克雷伯菌膜制剂的纯化B细胞免疫球蛋白分泌的影响，也获得了类似的结果。从这些数据可以得出结论，先前描述的Ci对免疫球蛋白分泌的增强作用可能是由T细胞介导的。
