Armstrong Lorene, Vaz Marcelo Gomes Marçal Vieira, Genuário Diego Bonaldo, Fiore Marli Fátima, Debonsi Hosana Maria
Physics and Chemistry Department, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, 14040-903, Brazil.
Department of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa, Paraná, Brazil.
Curr Microbiol. 2019 May;76(5):575-582. doi: 10.1007/s00284-019-01658-z. Epub 2019 Mar 13.
Cyanobacterial communities from the Brazilian Atlantic coast have been recently sampled through cultured and non-cultured approaches. The maintenance of cyanobacterial strains in laboratory cultures is an important source of material for biological and chemical evaluation as well as biotechnological investigations. In this way, this work aimed to identify, for the first time, by means of GC-MS analyses, the nonpolar chemical profiles of four morphologically distinct cyanobacterial strains: Cyanobium sp. CENA178, Cyanobium sp. CENA181, Oxynema sp. CENA135 and Nostoc sp. CENA175, which were previously isolated from Brazilian mangroves. Six distinct classes of volatile compounds were identified: acids, alcohols, fatty aldehydes, esters, ketones and aliphatic hydrocarbons, from which 12 compounds were detected. The predominant compounds were 1-octadecyne and tetradecanoic acid, obtained from Oxynema sp. CENA135 and; the last one being also observed in Cyanobium sp. CENA181. In addition, the aliphatic hydrocarbon heptadecane was produced by these cyanobacterial strains as well as by Nostoc sp. CENA175. The compounds produced by the studied cyanobacteria have already been reported as possessing pharmaceutical properties such as antioxidant, cytotoxic and antimicrobial activities, besides industrial importance as source of intermediates for biofuel production. It is also important to mention that, considering the number of non-identified compounds, which were not compatible with the searched databases, these strains are promising sources of new compounds, denoting the need for more studies. Accordingly, since these strains were isolated from saline or brackish waters, it is also expected that they might be cultivated in waters not used for human consumption, enabling a low-cost approach for biomass and metabolites production.
最近，通过培养和非培养方法对巴西大西洋沿岸的蓝藻群落进行了采样。在实验室培养中维持蓝藻菌株是进行生物学和化学评估以及生物技术研究的重要材料来源。通过这种方式，这项工作旨在首次通过气相色谱 - 质谱分析确定四种形态不同的蓝藻菌株的非极性化学特征：蓝藻属CENA178、蓝藻属CENA181、鞘丝藻属CENA135和念珠藻属CENA175，这些菌株先前是从巴西红树林中分离出来的。鉴定出了六类不同的挥发性化合物：酸、醇、脂肪醛、酯、酮和脂肪族烃，从中检测到了12种化合物。主要化合物是1 - 十八炔和十四烷酸，分别从鞘丝藻属CENA135中获得；十四烷酸在蓝藻属CENA181中也有发现。此外，这些蓝藻菌株以及念珠藻属CENA175都产生了脂肪族烃十七烷。所研究的蓝藻产生的化合物已被报道具有抗氧化、细胞毒性和抗菌活性等药用特性，此外作为生物燃料生产中间体的来源还具有工业重要性。还需要提及的是，考虑到与搜索数据库不匹配的未鉴定化合物数量，这些菌株是新化合物的有前景来源，这表明需要进行更多研究。因此，由于这些菌株是从盐水或微咸水中分离出来的，预计它们也可以在非人类消费用水中培养，从而实现低成本的生物质和代谢产物生产方法。
