单细胞基因组学。
Single-Cell Genomics.
机构信息
Division of Precision and Computational Diagnostics, Department of Clinical Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.
Computational Medicine Center, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA.
出版信息
Clin Chem. 2019 Aug;65(8):972-985. doi: 10.1373/clinchem.2017.283895. Epub 2019 Mar 14.
BACKGROUND
Single-cell genomics is an approach to investigate cell heterogeneity and to identify new molecular features correlated with clinical outcomes. This approach allows identification of the complexity of cell diversity in a sample without the loss of information that occurs when multicellular or bulk tissue samples are analyzed.
CONTENT
The first single-cell RNA-sequencing study was published in 2009, and since then many more studies and single-cell sequencing methods have been published. These studies have had a major impact on several fields, including microbiology, neurobiology, cancer, and developmental biology. Recently, improvements in reliability and the development of commercial single-cell isolation platforms are opening the potential of this technology to the clinical laboratory.
SUMMARY
In this review we provide an overview of the current state of single-cell genomics. We describe opportunities in clinical research and medical applications.
背景
单细胞基因组学是一种研究细胞异质性并鉴定与临床结果相关的新分子特征的方法。这种方法可以在不丢失信息的情况下，识别样本中细胞多样性的复杂性，而当对多细胞或批量组织样本进行分析时，信息会丢失。
内容
第一篇单细胞 RNA 测序研究于 2009 年发表，此后，又有更多的单细胞测序方法和研究发表。这些研究对包括微生物学、神经生物学、癌症和发育生物学在内的多个领域产生了重大影响。最近，可靠性的提高和商业单细胞分离平台的发展，为该技术在临床实验室中的应用开辟了潜力。
总结
在这篇综述中，我们概述了单细胞基因组学的现状。我们描述了在临床研究和医学应用中的机会。
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