Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region, China.
BGI Genomics, BGI-Shenzhen, Shenzhen, 518083, Guangdong, China.
Nat Commun. 2019 Mar 14;10(1):1216. doi: 10.1038/s41467-019-09142-9.
Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome of wild soybean accession W05, with a final assembled genome size of 1013.2 Mb and a contig N50 of 3.3 Mb. The analytical power of the W05 genome is demonstrated by several examples. First, we identify an inversion at the locus determining seed coat color during domestication. Second, a translocation event between chromosomes 11 and 13 of some genotypes is shown to interfere with the assignment of QTLs. Third, we find a region containing copy number variations of the Kunitz trypsin inhibitor (KTI) genes. Such findings illustrate the power of this assembly in the analysis of large structural variations in soybean germplasm collections. The wild soybean genome assembly has wide applications in comparative genomic and evolutionary studies, as well as in crop breeding and improvement programs.
高效的作物改良依赖于对不同种质资源中准确遗传信息的应用。在这里，我们报告了野生大豆品系 W05 的参考基因组，其最终组装基因组大小为 1013.2Mb，contig N50 为 3.3Mb。W05 基因组的分析能力通过几个例子得到了证明。首先，我们在决定驯化过程中种皮颜色的基因座上鉴定到一个倒位。其次，一些基因型的 11 号和 13 号染色体之间的易位事件表明其会干扰 QTL 的分配。第三，我们发现了一个包含 Kunitz 胰蛋白酶抑制剂 (KTI) 基因拷贝数变异的区域。这些发现说明了该组装在大豆种质资源中分析大的结构变异方面的强大功能。野生大豆基因组组装在比较基因组学和进化研究以及作物育种和改良计划中具有广泛的应用。
