Yang Xing, Hu Zhijuan, Yuan Jingjie, Zou Run, Wang Yilan, Peng Xuan, Xu Shan, Xie Chengjian
The Chongqing Key Laboratory of Molecular Biology of Plant Environmental Adaptations, Chongqing Normal University, Chongqing 401331, China.
Chongqing Engineering Research Center of Specialty Crop Resources, The College of Life Science, Chongqing Normal University, Chongqing 401331, China.
J Fungi (Basel). 2023 Oct 21;9(10):1037. doi: 10.3390/jof9101037.
, a virulent soil-borne fungus, elicits Verticillium wilt in numerous dicotyledonous plants through intricate pathogenic mechanisms. Ubiquitination, an evolutionarily conserved post-translational modification, marks and labels proteins for degradation, thereby maintaining cellular homeostasis. Within the ubiquitination cascade, ubiquitin ligase E3 demonstrates a unique capability for target protein recognition, a function often implicated in phytopathogenic virulence. Our research indicates that two ubiquitin ligase E3s, VdBre1 and VdHrd1, are intrinsically associated with virulence. Our findings demonstrate that the deletion of these two genes significantly impairs the ability of to colonize the vascular bundles of plants and to form typical penetration pegs. Furthermore, transcriptomic analysis suggests that VdBre1 governs the lipid metabolism pathway, while VdHrd1 participates in endoplasmic-reticulum-related processes. Western blot analyses reveal a significant decrease in histone ubiquitination and histone H3K4 trimethylation levels in the mutant. This research illuminates the function of ubiquitin ligase E3 in and offers fresh theoretical perspectives. Our research identifies two novel virulence-related genes and partially explicates their roles in virulence-associated structures and gene regulatory pathways. These findings augment our understanding of the molecular mechanisms inherent to .
一种致病力强的土传真菌，通过复杂的致病机制在众多双子叶植物中引发黄萎病。泛素化是一种进化上保守的翻译后修饰，它标记蛋白质以便降解，从而维持细胞内稳态。在泛素化级联反应中，泛素连接酶E3展现出对靶蛋白识别的独特能力，这一功能常与植物病原菌的毒力相关。我们的研究表明，两种泛素连接酶E3，即VdBre1和VdHrd1，与毒力内在相关。我们的研究结果表明，这两个基因的缺失显著损害了该真菌定殖植物维管束和形成典型穿透栓的能力。此外，转录组分析表明，VdBre1调控脂质代谢途径，而VdHrd1参与内质网相关过程。蛋白质免疫印迹分析显示，该真菌突变体中组蛋白泛素化和组蛋白H3K4三甲基化水平显著降低。本研究阐明了泛素连接酶E3在该真菌中的功能，并提供了新的理论视角。我们的研究鉴定出两个新的与毒力相关的基因，并部分阐明了它们在毒力相关结构和基因调控途径中的作用。这些发现加深了我们对该真菌内在分子机制的理解。
