Wang Chuan-Hui, Shi Hui-Hua, Chen Lin-Hui, Li Xiao-Li, Cao Guo-Liang, Hu Xiao-Feng
Department of Geriatrics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Neurology, Zhejiang Hospital, Zhejiang University, Hangzhou, China.
Front Genet. 2019 Feb 28;10:123. doi: 10.3389/fgene.2019.00123. eCollection 2019.
Atherosclerosis is one of the most common type of cardiovascular disease and the prime cause of mortality in the aging population worldwide. However, the detail mechanisms and special biomarkers of atherosclerosis remain to be further investigated. Lately, long non-coding RNAs (lncRNAs) has attracted much more attention than other types of ncRNAs. In our work, we found and confirmed differently expressed lncRNAs and mRNAs in atherosclerosis by analyzing GSE28829. We performed the weighted gene co-expression network analysis (WGCNA) by analyzing GSE40231 to confirm highly correlated genes. Gene Ontology (GO) analysis were utilized to assess the potential functions of differential expressed lncRNAs in atherosclerosis. Co-expression networks were also constructed to confirm hub lncRNAs in atherosclerosis. A total of 5784 mRNAs and 654 lncRNAs were found to be dysregulated in the progression of atherosclerosis. A total of 15 lncRNA-mRNA co-expression modules were identified in this study based on WGCNA analysis. Moreover, a few lncRNAs, such as ZFAS1, LOC100506730, LOC100506691, DOCK9-AS2, RP11-6I2.3, LOC100130219, were confirmed as important lncRNAs in atherosclerosis. Taken together, bioinformatics analysis revealed these lncRNAs were involved in regulating the leukotriene biosynthetic process, gene expression, actin filament organization, t-circle formation, antigen processing, and presentation, interferon-gamma-mediated signaling pathway, and activation of GTPase activity. We believed that this study would provide potential novel therapeutic and prognostic targets for atherosclerosis.
动脉粥样硬化是最常见的心血管疾病类型之一，也是全球老年人口死亡的主要原因。然而，动脉粥样硬化的具体机制和特殊生物标志物仍有待进一步研究。最近，长链非编码RNA（lncRNAs）比其他类型的非编码RNA受到了更多关注。在我们的研究中，通过分析GSE28829，我们发现并证实了动脉粥样硬化中lncRNAs和mRNAs的差异表达。通过分析GSE40231进行加权基因共表达网络分析（WGCNA），以确认高度相关的基因。利用基因本体论（GO）分析来评估差异表达的lncRNAs在动脉粥样硬化中的潜在功能。还构建了共表达网络以确认动脉粥样硬化中的关键lncRNAs。在动脉粥样硬化进展过程中，共发现5784个mRNA和654个lncRNAs表达失调。基于WGCNA分析，本研究共鉴定出15个lncRNA-mRNA共表达模块。此外，一些lncRNAs，如ZFAS1、LOC100506730、LOC100506691、DOCK9-AS2、RP11-6I2.3、LOC100130219，被确认为动脉粥样硬化中的重要lncRNAs。综上所述，生物信息学分析表明这些lncRNAs参与调节白三烯生物合成过程、基因表达、肌动蛋白丝组织、t环形成、抗原加工和呈递、干扰素-γ介导的信号通路以及GTPase活性的激活。我们相信这项研究将为动脉粥样硬化提供潜在的新型治疗和预后靶点。
