Feizabadi Narjes, Sarrafzadeh Javad, Fathali Mojtaba, Vasaghi-Gharamaleki Behnoosh, Dadgoo Mahdi, Kazemian Hossein, Kardan-Yamchi Jalil, Shariati Sonia Hesam
Department of Physiotherapy, School of Rehabilitation, Iran University of Medical Sciences, Tehran, Iran.
Surgeon and Specialist in Ear, Nose and Troat, School of Rehabilitation, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2018 Dec;10(6):354-360.
Bacterial pathogens, in particular drug resistant strains, involved in chronic rhinosinusitis may result in treatment failure. Ultrasound waves are able to destroy bacterial population in sinus cavities and can recover patients.
Twelve patients with chronic sinusitis and 10 healthy controls were treated by continuous ultrasound waves. Clinical specimens were collected before and after treatment. Serial diluted specimens were cultured on blood agar, chocolate and MacConkey agar plates for bacterial isolation. Bacterial DNA was extracted and used for detection using quantitative PCR.
was the most isolated bacterium (10 patients), which was eradicated from 8 patients after treatment. Using phenotypic methods at the beginning, 3 out of 10 healthy individuals were found to be positive. From 11 positive patients for identified by real time qPCR, 9 showed significant reduction after treatment. In the healthy group, was detected in 4 samples using qPCR, but they were clean at the second sampling.
According to our phenotypic and molecular experiments, continuous ultrasound treatment effectively reduced the bacterial population in studied patients (p < 0.01). This was a hopeful basis for doing more studies with ultrasound therapy as a treatment option.
慢性鼻窦炎中涉及的细菌病原体，尤其是耐药菌株，可能导致治疗失败。超声波能够破坏鼻窦腔内的细菌群体并使患者康复。
对12例慢性鼻窦炎患者和10例健康对照者进行连续超声波治疗。在治疗前后收集临床标本。将系列稀释后的标本接种于血琼脂、巧克力琼脂和麦康凯琼脂平板上进行细菌分离培养。提取细菌DNA并用于定量PCR检测。
是最常分离出的细菌（10例患者），治疗后8例患者的该细菌被根除。最初采用表型方法检测时，10名健康个体中有3名呈阳性。通过实时定量PCR鉴定出的11例阳性患者中，9例在治疗后细菌数量显著减少。在健康组中，通过定量PCR在4份样本中检测到 ，但第二次采样时样本均为阴性。
根据我们的表型和分子实验，连续超声波治疗有效减少了研究患者体内的细菌数量（p < 0.01）。这为将超声波治疗作为一种治疗选择开展更多研究提供了有希望的基础。
