Laboratory of Inorganic Chemistry and Advanced Materials, Department of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece; Laboratory of Materials for Electrotechnics, Department of Electrical and Computer Engineering, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
Laboratory of Inorganic Chemistry and Advanced Materials, Department of Chemical Engineering, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece; Department of Chemistry, University of Cape Town, Rondebosch 7700, Cape Town, South Africa.
J Inorg Biochem. 2019 May;194:180-199. doi: 10.1016/j.jinorgbio.2018.12.005. Epub 2019 Jan 2.
The quest for effective treatments of oxidative stress has concentrated over the years on new nanomaterials with improved antioxidant and antiradical activity, thereby attracting broad research interest. In that regard, research efforts in our lab were launched to pursue such hybrid materials involving a) synthesis of silica gel matrices, b) evaluation of the suitability of atoxic matrices as potential carriers for the controlled release of V(IV)(VOSO), V(V)(NaVO) compounds and a newly synthesized heterometallic lithium-vanadium(IV,V) tetranuclear compound containing vanadium-bound hydroxycarboxylic 1,3-diamine-2-propanol-N,N,N',N'-tetraacetic acid (DPOT), and c) investigation of structural and textural properties of silica nanoparticles (NPs) by different and complementary characterization techniques, inquiring into the nature of the encapsulated vanadium species and their interaction with the siloxane matrix, collectively targeting novel antioxidant and antiradical nanomaterials biotechnology. The physicochemical characterization of the vanadium-loaded SiO NPs led to the formulation of optimized material configuration linked to the delivery of the encapsulated antioxidant-antiradical load. Entrapment and drug release studies showed a) the competence of hybrid nanoparticles with respect to encapsulation efficiency of the vanadium compound (concentration dependence), b) congruence with the physicochemical features determined, and c) a well-defined release profile of NP load. Antioxidant properties and the free radical scavenging capacity of the new hybrid materials (containing VOSO, NaVO, and V-DPOT) were demonstrated through a) 2-diphenyl-1-picrylhydrazyl (DPPH) free radical, and b) intracellular-extracellular reactive oxygen species (ROS) assays, through UV-Visible spectroscopy techniques, collectively showing that the hybrid silica NPs (empty-loaded) could serve as an efficient platform for nanodrug formulations counteracting oxidative stress.
多年来，人们一直在寻找有效的氧化应激治疗方法，重点是开发具有改进的抗氧化和抗自由基活性的新型纳米材料，从而引起了广泛的研究兴趣。在这方面，我们实验室的研究工作旨在寻求涉及以下方面的混合材料：(a)合成硅胶基质，(b)评估无毒基质作为控制释放 V(IV)(VOSO)、V(V)(NaVO)化合物和新合成的含钒结合羟羧酸 1,3-二胺-2-丙醇-N,N,N',N'-四乙酸（DPOT）的异金属锂-钒(IV,V)四核化合物的潜在载体的适宜性，以及 (c)通过不同和互补的表征技术研究硅胶纳米粒子（NPs）的结构和结构特性，研究包裹钒物种的性质及其与硅氧烷基质的相互作用，共同针对新型抗氧化和抗自由基纳米生物技术。负载钒的 SiO2 NPs 的物理化学特性导致了优化的材料结构的制定，该结构与包裹的抗氧化-抗自由基负载的传递有关。包埋和药物释放研究表明：(a)混合纳米粒子在包裹钒化合物的效率（浓度依赖性）方面具有竞争力，(b)与所确定的物理化学特征一致，以及 (c)NP 负载的明确释放曲线。新的混合材料（含有 VOSO、NaVO 和 V-DPOT）的抗氧化特性和自由基清除能力通过 a）2-二苯基-1-苦基肼基（DPPH）自由基和 b）细胞内-细胞外活性氧（ROS）测定，通过紫外可见光谱技术，共同表明混合硅胶 NPs（空负载）可用作对抗氧化应激的纳米药物制剂的有效平台。
