Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2019 Mar 14;20(6):1276. doi: 10.3390/ijms20061276.
is a gynomonoecious plant that bears both female zygomorphic ray florets and bisexual actinomorphic disc florets in the inflorescence. This sexual system is quite prevalent in Asteraceae, but poorly understood. CYCLOIDEA (CYC) 2 subclade transcription factors, key regulators of flower symmetry and floret identity in Asteraceae, have also been speculated to function in reproductive organs and could be an entry point for studying gynomonoecy. However, the molecular mechanism is still unclear. On the other hand, the WUSCHEL (WUS) transcription factor has been proven to play a vital role in the development of reproductive organs. Here, a homologue () in was isolated and characterized. Overexpression of in led to shorter siliques and fewer stamens, which was similar to -like genes reported before. In addition, both and were highly expressed in flower buds during floral organ differentiation and in the reproductive organs at later development stages, indicating their involvement in the development of reproductive organs. Moreover, CmWUS could directly interact with CmCYC2d. Thus, our data suggest a collaboration between CmWUS and CmCYC2 in the regulation of reproductive organ development in chrysanthemum and will contribute to a further understanding of the gynomonoecious sexual system in Asteraceae.
是一种雌雄同体的植物，在花序中同时具有雌性辐射状的舌状花和两性辐射状的盘状花。这种性系统在菊科中相当普遍，但了解甚少。拟南芥 CYCLOIDEA（CYC）2 亚科转录因子是菊科花对称性和小花身份的关键调节因子，也被推测在生殖器官中起作用，可能是研究雌雄同体的切入点。然而，其分子机制尚不清楚。另一方面，WUSCHEL（WUS）转录因子已被证明在生殖器官发育中起着至关重要的作用。在这里，分离并鉴定了 的同源物()。在 中过表达 导致短的果荚和较少的雄蕊，这与之前报道的 样基因相似。此外，和 在花器官分化期间的花蕾中和后期发育阶段的生殖器官中高度表达，表明它们参与了生殖器官的发育。此外，CmWUS 可以直接与 CmCYC2d 相互作用。因此，我们的数据表明 CmWUS 和 CmCYC2 在菊花生殖器官发育的调控中存在协作关系，并将有助于进一步了解菊科中的雌雄同体性系统。
