Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa, 16059, Turkey.
Beekeeping Development-Application and Research Center, Uludag University, Gorukle/Bursa, 16059, Turkey.
Cryobiology. 2019 Apr;87:28-31. doi: 10.1016/j.cryobiol.2019.03.005. Epub 2019 Mar 13.
The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thawing quality of drone sperm. Semen samples were collected from sexually mature drones. Pooled semen was diluted with extender without RJ (control) or supplemented with different concentrations of RJ (1, 2, 4 or 8%). Sperm motility, plasma membrane functional integrity, and acrosomal integrity were evaluated. At post thaw, the highest sperm motility and acrosomal integrity rates were obtained in the RJ1 group. Functional integrity of sperm membrane was better preserved in the RJ1 and RJ2 groups compare to the other groups. The study shows that RJ supplemented extenders have beneficial effects on drone semen parameters. The results of the present study demonstrated advantage of using 1% RJ supplemented extender.
本研究旨在评估不同浓度的蜂王浆（RJ）对雄蜂精子解冻后质量的影响。从性成熟的雄蜂中采集精液样本。将混合精液用不含 RJ 的稀释液（对照）或添加不同浓度的 RJ（1、2、4 或 8%）的稀释液稀释。评估精子活力、质膜功能完整性和顶体完整性。解冻后，RJ1 组精子活力和顶体完整性最高。与其他组相比，RJ1 和 RJ2 组的精子质膜功能完整性更好地保存。研究表明，RJ 补充剂对雄蜂精液参数有有益的影响。本研究结果表明，使用 1%RJ 补充剂的优势。
