Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza 12618, Egypt.
Cells. 2022 Oct 27;11(21):3403. doi: 10.3390/cells11213403.
Bee bread has numerous nutritional benefits and bioactive compounds. Other bee byproducts have been used as extender additives to improve semen cryopreservation. Here, we examined the effects of supplementing egg yolk extender (EYE) or soybean lecithin extender (SBLE) with bee bread extract (BBE) on the quality of cryopreserved ram semen. Semen was collected from five adult Rahmani rams once a week for 7 weeks. EYE and SBLE were supplemented with BBE. Antioxidant capacity and total phenolic compound, total flavonoid compound, and total soluble carbohydrate levels of BBE were measured. Sperm characteristics, including progressive motility, viability, abnormalities, membrane integrity, and acrosome integrity, were analyzed after equilibration, thawing, and thawing followed by a 2-h incubation. The total antioxidant capacity and malondialdehyde, hydrogen peroxide, aspartate transaminase, alanine transaminase, alkaline phosphatase, and total acid phosphatase levels in extenders were determined after thawing. Sperm apoptosis was analyzed using annexin V assays. SBLE was more effective than EYE for cryopreserving ram semen. Extender supplementation with BBE improved ram semen quality during freezing in a concentration-dependent pattern. Motility, vitality, and membrane integrity were particularly enhanced in BBE-treated semen. Additionally, BBE promoted antioxidant and enzymatic activities and reduced apoptosis in semen. Thus, extender supplementation with BBE improved sperm cryopreservation.
蜂粮具有许多营养价值和生物活性化合物。其他蜂产品已被用作 extender 添加剂，以改善精液的冷冻保存。在这里，我们研究了在蛋黄液 extender (EYE) 或大豆卵磷脂 extender (SBLE) 中添加蜂粮提取物 (BBE) 对冷冻公羊精液质量的影响。每周从 5 只成年 Rahmani 公羊中采集一次精液，持续 7 周。EYE 和 SBLE 补充了 BBE。测量了 BBE 的抗氧化能力和总酚化合物、总黄酮化合物和总可溶性碳水化合物水平。在平衡、解冻以及解冻后 2 小时孵育后，分析了精子特征，包括前向运动、活力、异常、膜完整性和顶体完整性。测定了解冻后 extender 中的总抗氧化能力和丙二醛、过氧化氢、天门冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶和总酸性磷酸酶水平。使用 annexin V 测定法分析精子凋亡。与 EYE 相比，SBLE 更有效地冷冻公羊精液。以浓度依赖的方式在 extender 中添加 BBE 可提高公羊精液在冷冻过程中的质量。运动性、活力和膜完整性在 BBE 处理的精液中得到特别改善。此外，BBE 促进了精液中的抗氧化和酶活性，并减少了凋亡。因此，在 extender 中添加 BBE 可改善精子的冷冻保存。
