Department of Entomology, China Agricultural University, Beijing, China; Department of Entomology, Purdue University, West Lafayette, IN, USA.
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, Henan, China.
Mol Phylogenet Evol. 2019 Jun;135:123-135. doi: 10.1016/j.ympev.2019.03.005. Epub 2019 Mar 12.
Phylogenetic analysis based on mitochondrial genomic data from 25 stonefly species recovered a well-supported tree resolving higher-level relationships within Plecoptera (stoneflies). The monophyly of both currently recognized suborders was strongly supported, concordant with previous molecular analyses of Plecoptera. The southern hemisphere suborder Antarctoperlaria formed two clades: Eustheniidae + Diamphipnoidae and Austroperlidae + Gripopterygidae; consistent with relationships proposed based on morphology. The largely northern hemisphere suborder Arctoperlaria also divided into two groups, Euholognatha and Systellognatha, each composed of the five families traditionally assigned to each infraorder (the placement Scopuridae by mt genome data remains untested at this time). Within Euholognatha, strong support for the clade Nemouridae + Notonemouridae confirmed the northern origin of the currently southern hemisphere restricted Notonemouridae. Other family level relationships within the Arctoperlaria differ from those recovered by previous morphology and molecular based analyses. A fossil-calibrated divergence estimation suggests the formation of two suborders dates back to the Jurassic (181 Ma), with subsequent diversification of most stonefly families during the Cretaceous. This result confirms the hypothesis that initial divergence between the suborders was driven by the breakup of the supercontinent Pangaea into Laurasia and Gondwanaland (commencing 200 Ma and complete by 150 Ma).
基于 25 种石蝇物种的线粒体基因组数据进行的系统发育分析，重建了一个支持度较高的树，解决了石蝇目中的高级分类关系。目前公认的两个亚目的单系性得到了强烈支持，与石蝇目之前的分子分析一致。南半球的亚目 Antarctoperlaria 形成了两个分支：Eustheniidae + Diamphipnoidae 和 Austroperlidae + Gripopterygidae；与基于形态学提出的关系一致。主要分布在北半球的亚目 Arctoperlaria 也分为两个组，Euholognatha 和 Systellognatha，每组由五个传统上分配给每个亚目的科组成（目前南半球的 Notonemouridae 由 mt 基因组数据分配仍然未经测试）。在 Euholognatha 中，Nemouridae + Notonemouridae 分支的强烈支持证实了目前仅限于南半球的 Notonemouridae 的北方起源。Arctoperlaria 内的其他科级关系与以前的形态学和基于分子的分析结果不同。化石校准的分歧估计表明，两个亚目的形成可以追溯到侏罗纪（1.81 亿年前），随后大多数石蝇科在白垩纪多样化。这一结果证实了这样一种假设，即亚目的最初分歧是由超大陆盘古大陆分裂为劳亚古陆和冈瓦纳大陆（始于 2 亿年前，1.5 亿年前完成）驱动的。
