Department of Pharmacy, Nanjing Second Hospital, Nanjing University of Chinese Medicine, Nanjing 210003, China.
Department of Pediatrics, The Taizhou People's Hospital, Taizhou 225300, China
Biosci Rep. 2019 Apr 5;39(4). doi: 10.1042/BSR20181854. Print 2019 Apr 30.
Accumulating evidence suggests that miRNAs play a crucial role in the development of prostate cancer (PC); however, the role of miR-500 in PC remains poorly understood. The data presented here reveal abnormal increases in miR-500 expression in PC tissues and cell lines. Suppression of miR-500 expression significantly inhibited the proliferation of PC-3 and LnCap cells and was negatively regulative with low-density lipoprotein receptor-related protein 1B (LRP1B). Increased cell cycle arrest at the G1 stage and decreased protein expression of cyclinD1 and CDK2 was observed in response to miR-500 knockdown in PC-3 and LnCap cells, in combination with LRP1B overexpression. LRP1B was identified as a target of miR-500 and was significantly decreased in PC tissues. Taken together, these findings demonstrate that miR-500 plays an important role in the proliferation of PC cells via the inhibition of LRP1B expression.
越来越多的证据表明 miRNAs 在前列腺癌（PC）的发展中起着至关重要的作用；然而，miR-500 在 PC 中的作用仍知之甚少。这里呈现的数据显示 miR-500 在 PC 组织和细胞系中的表达异常增加。抑制 miR-500 的表达显著抑制了 PC-3 和 LnCap 细胞的增殖，并且与低密度脂蛋白受体相关蛋白 1B（LRP1B）呈负相关。在 PC-3 和 LnCap 细胞中观察到 miR-500 敲低后细胞周期停滞在 G1 期增加，细胞周期蛋白 D1 和 CDK2 的蛋白表达减少，同时 LRP1B 过表达。LRP1B 被鉴定为 miR-500 的靶标，并且在 PC 组织中显著减少。综上所述，这些发现表明 miR-500 通过抑制 LRP1B 的表达在 PC 细胞的增殖中发挥重要作用。
