Laboratory of Regulation of Gene Expression, Institute of Cytology, Russian Academy of Sciences, 194064 St Petersburg, Russia
Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, 194064 St Petersburg, Russia.
J Exp Biol. 2019 Mar 21;222(Pt 6):jeb198556. doi: 10.1242/jeb.198556.
Echinoderms, possessing outstanding regenerative capabilities, provide a unique model system for the study of response to injury. However, little is known about the proteomic composition of coelomic fluid, an important biofluid circulating throughout the animal's body and reflecting the overall biological status of the organism. In this study, we used LC-MALDI tandem mass spectrometry to characterize the proteome of the cell-free coelomic fluid of the starfish and to follow the changes occurring in response to puncture wound and blood loss. In total, 91 proteins were identified, of which 61 were extracellular soluble and 16 were bound to the plasma membrane. The most represented functional terms were 'pattern recognition receptor activity' and 'peptidase inhibitor activity'. A series of candidate proteins involved in early response to injury was revealed. Ependymin, β-microseminoprotein, serum amyloid A and avidin-like proteins, which are known to be involved in intestinal regeneration in the sea cucumber, were also identified as injury-responsive proteins. Our results expand the list of proteins potentially involved in defense and regeneration in echinoderms and demonstrate dramatic effects of injury on the coelomic fluid proteome.
棘皮动物具有出色的再生能力，为研究损伤反应提供了独特的模型系统。然而，对于体腔液（一种在动物体内循环并反映生物体整体生物学状态的重要生物流体）的蛋白质组组成知之甚少。在这项研究中，我们使用 LC-MALDI 串联质谱法对海星的无细胞体腔液的蛋白质组进行了表征，并跟踪了对穿刺伤和失血的反应中发生的变化。总共鉴定出 91 种蛋白质，其中 61 种是细胞外可溶性的，16 种与质膜结合。最具代表性的功能术语是“模式识别受体活性”和“肽酶抑制剂活性”。揭示了一系列涉及早期损伤反应的候选蛋白。已知参与海参肠道再生的脑啡肽、β-微精蛋白、血清淀粉样 A 和抗生物素蛋白样蛋白也被鉴定为对损伤有反应的蛋白。我们的结果扩展了潜在参与棘皮动物防御和再生的蛋白质列表，并证明了损伤对体腔液蛋白质组的显著影响。
