Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados UFGD, Dourados, Mato Grosso do Sul State, Brazil.
Faculty of Health Sciences, Federal University of Grande Dourados UFGD, Dourados, Mato Grosso do Sul State, Brazil.
Inflammation. 2019 Jun;42(3):1045-1055. doi: 10.1007/s10753-019-00966-7.
Palicourea crocea (Sw.) Roem. and Schult., "douradinha," are used by treat inflammation (edema). Croceaine A (PC-1) was isolated from P. crocea (MEPC) leaves and studied for its antioxidant and anti-inflammatory activity, as well as concentrations of constituents and acute toxicity. The phenols and polyphenolics compounds and HPLC/DAD were determined. The antioxidant activity were evaluated for DPPH, ABTS, and MDA. MEPC (300, 100, and 300 mg/kg) and PC-1 (10 and 30 mg/kg) were tested for anti-inflammatory effects in paw edema, pleurisy, cold sensitivity, and mechanical hyperalgesia. Acute toxicity is also described. MEPC contained high concentrations of phenolic and flavonoid compounds (≤ 800.35 mg/g), as well as caffeic acid, ferulic acid, rutin, and quercetin, revealed by HPLC-DAD analysis. MEPC displayed antioxidant activity against ABTS radicals (IC = 68.5 μg/mL) and MDA (74%). MEPC and alkaloid PC-1 demonstrated an anti-edematogenic effect in Cg-induced paw edema in 2 and 4 h, and also significantly reduced mechanical hyperalgesia, cold response to acetone in mice, at 3 and 4 h after injection, as well as leukocyte migrationin the pleurisy model. No toxicity was detected by MEPC. For the first time, P. crocea was evaluated for its antioxidant, systemic anti-inflammatory, and anti-hyperalgesic activities.
Palicourea crocea (Sw.) Roem. and Schult.，俗称“douradinha”，被用于治疗炎症（水肿）。Croceaine A（PC-1）是从 P. crocea（MEPC）叶子中分离出来的，研究其抗氧化和抗炎活性，以及成分浓度和急性毒性。测定了酚类和多酚化合物以及 HPLC/DAD。通过 DPPH、ABTS 和 MDA 评估了抗氧化活性。MEPC（300、100 和 300mg/kg）和 PC-1（10 和 30mg/kg）用于测试爪水肿、胸膜炎、冷敏感和机械性痛觉过敏的抗炎作用。还描述了急性毒性。MEPC 含有高浓度的酚类和类黄酮化合物（≤800.35mg/g），以及通过 HPLC-DAD 分析发现的咖啡酸、阿魏酸、芦丁和槲皮素。MEPC 对 ABTS 自由基（IC=68.5μg/mL）和 MDA（74%）具有抗氧化活性。MEPC 和生物碱 PC-1 在 Cg 诱导的爪水肿中在 2 和 4 小时显示出抗水肿作用，并且在注射后 3 和 4 小时还显著降低了机械性痛觉过敏、小鼠对丙酮的冷反应以及胸膜炎模型中的白细胞迁移。MEPC 未检测到毒性。这是首次对 P. crocea 的抗氧化、系统性抗炎和抗痛觉过敏活性进行评估。
