Piva Raul Cremonezi, Verdan Maria Helena, Branquinho Lidiane Schultz, Kassuya Cândida Aparecida Leite, Cardoso Claudia Andrea Lima
Postgraduate Program in Chemistry, Federal University of Grande Dourados (UFGD), Rodovia Dourados/Itahum, Km 12, Caixa Postal 364, 79804-970, Dourados, MS, Brazil.
Postgraduate Program in Health Sciences, Federal University of Grande Dourados (UFGD), Rodovia Dourados/Itahum, Km 12, Caixa Postal 364, 79804-970, Dourados, MS, Brazil.
J Ethnopharmacol. 2021 Jul 15;275:114136. doi: 10.1016/j.jep.2021.114136. Epub 2021 Apr 20.
The population uses the aqueous extract as tea from leaves of Ocimum selloi Benth. (alfavaca) for pain and inflammation issues. This study is motivated by a lack of data about inflammation properties of O. selloi.
This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO. OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol. Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice. The dose of 300 mg/kg of OSEO reduced cold hyperalgesia 4 h after carrageenan.
The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
当地居民将丁香罗勒（Ocimum selloi Benth.，也称alfavaca）的叶子制成水提取物当作茶饮用，用于缓解疼痛和炎症问题。本研究的开展是由于缺乏关于丁香罗勒炎症特性的数据。
本研究调查了从丁香罗勒叶子中提取的水提取物（OSAE）和精油（OSEO）在小鼠模型中的化学成分和抗炎活性。
对样品进行了抗氧化活性和总酚含量评估，而OSAE的化学成分通过超高效液相色谱-二极管阵列-电喷雾质谱联用仪（U-HPLC-DAD-ESI-MS）测定，OSEO的化学成分通过气相色谱-质谱联用仪（GC-MS）测定。在角叉菜胶诱导的胸膜炎、爪肿胀以及小鼠机械性痛觉过敏模型中，分别以30、100和300mg/kg的剂量对OSAE和OSEO进行口服给药测试。
在OSAE中鉴定出四种糖基化黄酮类化合物和一种有机酸，在OSEO中鉴定出九种物质，其中两种主要成分是反式茴香脑和甲基丁香酚。OSAE和OSEO的口服治疗显著抑制了雌性瑞士小鼠角叉菜胶诱导的胸膜炎，此外，OSAE和OSEO显著减轻了雄性瑞士小鼠角叉菜胶模型后1、2和4小时的爪肿胀、3和4小时的机械性痛觉过敏以及3小时的冷痛觉过敏。300mg/kg剂量的OSEO在角叉菜胶处理后4小时减轻了冷痛觉过敏。
结果证明了从丁香罗勒叶子中获得的两种物质（主要是OSAE）具有抗炎、抗水肿、抗痛觉过敏和抗伤害感受的潜力，支持了该物种的普遍用途。
