Mogard M H, Reeve J R, Shively J E, Ben-Avram C M, Eysselein V E, Walsh J H
Regul Pept. 1986 Jul;14(4):313-21. doi: 10.1016/0167-0115(86)90173-4.
Neurotensin-like immunoreactivity can be detected in extracts of canine upper gastrointestinal mucosa when measured by carboxyl terminal but not by amino terminal antibodies to neurotensin. The nature of this immunoreactive material was characterized by complete purification on gel filtration and HPLC followed by peptide microsequence analysis. The structure obtained was Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-(Leu), identical in structure to the carboxyl terminal decapeptide of neurotensin. It cannot, however, be excluded that this neurotensin decapeptide was generated from a larger neurotensin-like peptide during the extraction procedure by a physiological or artificial enzymatic process. Since carboxyl terminal neurotensin fragments containing eight or more residues have full biological activity, this peptide may be responsible for neurotensin-like biological activities within the mucosa of, or after release from, the upper gut.
当用神经降压素的羧基末端抗体而非氨基末端抗体进行检测时，在犬类上消化道黏膜提取物中可检测到神经降压素样免疫反应性。这种免疫反应性物质的性质通过凝胶过滤和高效液相色谱进行完全纯化，随后进行肽微序列分析来表征。所获得的结构为Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-(Leu)，其结构与神经降压素的羧基末端十肽相同。然而，不能排除这种神经降压素十肽是在提取过程中通过生理或人工酶促过程从更大的神经降压素样肽产生的。由于含有八个或更多残基的羧基末端神经降压素片段具有完全的生物活性，这种肽可能是上消化道黏膜内或从上消化道释放后神经降压素样生物活性的原因。
