Institute of Clinical Epidemiology and Evidence-Based Medicine, Tongji University School of Medicine, Shanghai, China.
Key Laboratory of Arrhythmias of the Ministry of Education of China, Tongji University School of Medicine, Shanghai, China.
Acta Biochim Biophys Sin (Shanghai). 2019 Apr 1;51(4):422-430. doi: 10.1093/abbs/gmz018.
Angiotensin II (AII) has been well known to induce cardiomyocyte hypertrophy. Epigallocatechin-3-gallate (EGCG) is the main active component of green tea and it has been shown to exhibit strong cardioprotective potential, although the underlying molecular mechanisms remain unclear. In this study, we investigated the role and mechanism of EGCG in preventing AII-induced cardiomyocyte hypertrophy using rat H9c2 cardiomyocytes cells. Reactive oxygen species assay, cell size, and mRNA expression of cardiac hypertrophy markers ANP and BNP were assessed in response to AII treatment. In addition, expression of proteins involved in Hippo signaling pathway were determined by western blot analysis. We found that AII treatment resulted in significant upregulation of ANP and BNP expression levels and increase in H9c2 cell size, which were markedly attenuated by EGCG treatment. Furthermore, our results suggested that EGCG inhibited AII-induced cardiac hypertrophy via regulating the Hippo signaling pathway. Therefore, EGCG may be an effective agent for preventing cardiac hypertrophy.
血管紧张素 II（AII）已被证实可诱导心肌细胞肥大。表没食子儿茶素没食子酸酯（EGCG）是绿茶的主要活性成分，已被证明具有很强的心脏保护潜力，尽管其潜在的分子机制尚不清楚。在这项研究中，我们使用大鼠 H9c2 心肌细胞研究了 EGCG 在预防 AII 诱导的心肌细胞肥大中的作用和机制。通过活性氧测定、细胞大小和心脏肥大标志物 ANP 和 BNP 的 mRNA 表达评估来响应 AII 处理。此外，通过 Western blot 分析测定了涉及 Hippo 信号通路的蛋白质的表达。我们发现，AII 处理导致 ANP 和 BNP 表达水平的显著上调和 H9c2 细胞大小的增加，而 EGCG 处理明显减弱了这种作用。此外，我们的结果表明，EGCG 通过调节 Hippo 信号通路抑制 AII 诱导的心肌肥大。因此，EGCG 可能是预防心肌肥大的有效药物。
