Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China.
Department of Clinical Laboratory, The second Hospital of Shandong University, Jinan, Shandong, 250033, China.
Biomed Pharmacother. 2019 May;113:108758. doi: 10.1016/j.biopha.2019.108758. Epub 2019 Mar 14.
Colon cancer-associated transcript 2 (CCAT2) plays a crucial role in several cancers. However, the clinical significance of circulating CCAT2 expression in colorectal cancer (CRC) has not previously been elucidated. In this study, we aimed to elucidate the potential role of CCAT2 and its clinical significance of circulating expression level in CRC.
We detected the expression of CCAT2 in 75 pairs of tumorous and adjacent non-tumorous tissues derived from CRC patients by quantitative real-time polymerase chain reaction. The serum levels of CCAT2 expression were detected in an independent cohort of healthy controls and CRC patients. We analyzed the relationship between CCAT2 levels in serum and clinicopathological features of CRC patients. We also compared CCAT2 levels in paired pre-operative and post-operative serum samples. Furthermore, the existence of serum CCAT2 in exosomes was investigated.
The levels of CCAT2 expression were significantly over-expressed in tumor tissues (p < 0.05) compared to adjacent non-tumorous tissues. Higher CCAT2 expression was associated with advanced CRC patients. Moreover, the serum levels of CCAT2 expression were significantly over-expressed in CRC patients (p < 0.05) compared to those in healthy subjects. In addition, the CCAT2 levels were significantly decreased in post-operative samples than those in pre-operative ones (P = 0.01). We also found that CCAT2 expression was up-regulated in CRC exosomes (P < 0.001) and no significant differences of CCAT2 levels were found between in serum and in exosomes.
Our data indicate that circulating CCAT2 which might protected by exosomes can serve as a novel potential predictor in CRC.
结肠癌相关转录物 2（CCAT2）在几种癌症中发挥着关键作用。然而，循环 CCAT2 表达在结直肠癌（CRC）中的临床意义尚未阐明。在本研究中，我们旨在阐明 CCAT2 的潜在作用及其在 CRC 中循环表达水平的临床意义。
我们通过实时定量聚合酶链反应检测了 75 对来自 CRC 患者的肿瘤和相邻非肿瘤组织中的 CCAT2 表达。在独立的健康对照者和 CRC 患者队列中检测了 CCAT2 的血清表达水平。我们分析了 CCAT2 水平与 CRC 患者临床病理特征之间的关系。我们还比较了配对术前和术后血清样本中的 CCAT2 水平。此外，还研究了血清 CCAT2 在 exosomes 中的存在情况。
与相邻非肿瘤组织相比，肿瘤组织中 CCAT2 的表达水平显著上调（p < 0.05）。更高的 CCAT2 表达与晚期 CRC 患者相关。此外，CRC 患者的血清 CCAT2 表达水平明显高于健康对照组（p < 0.05）。此外，术后样本中的 CCAT2 水平明显低于术前样本（P = 0.01）。我们还发现 CRC exosomes 中 CCAT2 表达上调（P < 0.001），并且血清和 exosomes 中 CCAT2 水平没有明显差异。
我们的数据表明，可能受 exosomes 保护的循环 CCAT2 可作为 CRC 的一种新的潜在预测因子。
