School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China.
School of Functional Food and Wine, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, 110016, China.
J Ethnopharmacol. 2019 Jun 12;237:28-38. doi: 10.1016/j.jep.2019.03.029. Epub 2019 Mar 14.
Alpinia oxyphylla-Schisandra chinensis herb pair (ASHP), composed of Alpinia oxyphylla Miq. Fructus (Yizhi, in Chinese) and Schisandra chinensis (Turcz.) Baill Fructus (Wuweizi, in Chinese) has been used in many traditional Chinese prescriptions such as Yizhi Wuwei pill and Jiannao pill.
This study was primarily dealt with studying the effects of Alpinia oxyphylla-Schisandra chinensis herb pair (ASHP) on learning and cognitive impairment in the Aβ induced mouse model.
The chemical composition quantitative analysis was by UPLC. Then the Y maze and Morris water maze test were used to determine the capability of ASHP extracts on improving memory. Histological changes and apoptotic features were detected by HE staining and TUNEL staining, respectively. qPCR was used to detect the changes in the mRNA of caspase3, caspase8 and caspase9 and western-blot was used to detect the changes in the levels of cleaved-caspase3, cleaved-caspase8 and cleaved-caspase9. The levels of some inflammatory factors such as IKK, IκB and NF-κB; anti-apoptotic factors such as bcl-2, bcl-xl, pro-apoptotic factors including bad, bax, p53 were assessed via immunohistochemistry (IHC) and western-blot.
Administration of ASHP extracts had higher spontaneous alternation ratio in the Y maze, more quadrant dwell time and shorter escape latency compared with model group in the Morris water maze. ASHP treated groups significantly inhibited NF-κB pathway and apoptosis-related pathway in the hippocampus.
This study demonstrated that ASHP had the ability to ameliorate abnormal changes in cognitive behavior, biochemical and histopathology induced by Aβ in the mouse model. The powerful role of ASHP is to inhibit the NF-κB inflammatory signaling pathway and cut down the damage of apoptosis. This study revealed ASHP might be a potential therapy for cognitive and behavioral deficits.
益智-五味子药对由益智（益智仁，中药名）和五味子（北五味子，中药名）组成，在许多中药方剂中都有应用，如益智五味丸和健脑丸。
本研究主要研究益智-五味子药对对 Aβ诱导的小鼠模型学习和认知障碍的影响。
采用 UPLC 进行化学成分定量分析。然后通过 Y 迷宫和 Morris 水迷宫实验来确定益智-五味子提取物改善记忆的能力。通过 HE 染色和 TUNEL 染色分别检测组织学变化和细胞凋亡特征。采用 qPCR 检测 caspase3、caspase8 和 caspase9mRNA 的变化，采用 Western blot 检测 cleaved-caspase3、cleaved-caspase8 和 cleaved-caspase9 水平的变化。通过免疫组化（IHC）和 Western blot 评估 IKK、IκB 和 NF-κB 等炎症因子、bcl-2、bcl-xl 等抗凋亡因子以及 bad、bax、p53 等促凋亡因子的水平。
与模型组相比，益智-五味子提取物给药组在 Y 迷宫中的自发交替率更高，在 Morris 水迷宫中的象限停留时间更长，逃避潜伏期更短。益智-五味子处理组显著抑制了海马 NF-κB 通路和凋亡相关通路。
本研究表明，益智-五味子具有改善 Aβ 诱导的小鼠模型认知行为、生化和组织病理学异常变化的能力。益智-五味子的强大作用是抑制 NF-κB 炎症信号通路，减少细胞凋亡的损伤。本研究揭示了益智-五味子可能是一种治疗认知和行为缺陷的潜在疗法。
