整合全长转录组和花青素代谢物分析以了解……果实着色机制
Integration of full-length transcriptomes and anthocyanin metabolite analysis for understanding fruit coloration mechanism in .
作者信息
Li Haiyan, Fan Chunxue, Liu Jiushi, Wang Biao, Li Hongbo
机构信息
College of Horticulture, Shenyang Agricultural University, Shenyang, 110866 China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193 China.
出版信息
Physiol Mol Biol Plants. 2022 May;28(5):921-933. doi: 10.1007/s12298-022-01179-3. Epub 2022 May 12.
UNLABELLED
Coloration directly affects the commercial value of fruits. The composition and content of anthocyanin determine the fruit coloration. However, the molecular mechanism of anthocyanin biosynthesis and regulation in this fruit remains unknown. In this study, we performed integrative full-length transcriptomics and targeted metabolomics analyses in fruits at four different developmental stages to elucidate the coloration mechanism. Cyanidin3--xyl-rutinoside is the key anthocyanin, which is responsible for the reddening of fruits, and its accumulation gradually accelerated from the 80th day after fluorescence. Overall, 122,289 unigenes with an average length of 2592 bp and an N50 of 4232 bp were obtained through single-molecule real-time sequencing; a total of 16,456 differentially expressed genes were identified. Moreover, 10 full-length structural genes related to anthocyanin biosynthesis were found to be significantly differentially expressed with fruit ripening. Moreover, 10 glycosyltransferases (GTs) that may possess the activities of anthocyanidin 3--glucosyltransferase, anthocyanidin 3--glucoside rhamnosyltransferase, and xylosyltransferases, which are involved in the final three steps for cyanidin3--xyl-rutinoside synthesis, were identified through phylogenetic analysis. Based on these findings, we constructed the complete anthocyanin biosynthetic pathway in fruits; five s, three s, and two s potentially involved in regulating anthocyanin biosynthesis in fruits were also selected. Our study provides the foundation for further research on the molecular mechanism of anthocyanin biosynthesis and regulation for improving the quality of fruits. The results of full-length transcriptomes would provide researchers with novel insights into the molecular cloning of enzymes and their activity.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12298-022-01179-3.
未标记
色泽直接影响果实的商业价值。花青素的组成和含量决定果实的色泽。然而，该果实中花青素生物合成和调控的分子机制仍不清楚。在本研究中，我们对处于四个不同发育阶段的果实进行了综合全长转录组学和靶向代谢组学分析，以阐明色泽形成机制。矢车菊素3 - 木糖 - 芸香糖苷是关键的花青素，它导致果实变红，其积累从开花后第80天开始逐渐加速。总体而言，通过单分子实时测序获得了122,289个单基因，平均长度为2592 bp，N50为4232 bp；共鉴定出16,456个差异表达基因。此外，发现10个与花青素生物合成相关的全长结构基因随果实成熟而显著差异表达。此外，通过系统发育分析鉴定出10种糖基转移酶（GTs），它们可能具有矢车菊素3 - 葡萄糖基转移酶、矢车菊素3 - 葡萄糖苷鼠李糖基转移酶和木糖基转移酶的活性，这些酶参与矢车菊素3 - 木糖 - 芸香糖苷合成的最后三个步骤。基于这些发现，我们构建了该果实完整的花青素生物合成途径；还筛选出了5个、3个和2个可能参与调控该果实花青素生物合成的基因。我们的研究为进一步研究花青素生物合成和调控的分子机制以提高该果实品质奠定了基础。全长转录组的结果将为研究人员提供关于酶分子克隆及其活性的新见解。
补充信息
在线版本包含可在10.1007/s12298 - 022 - 01179 - 3获取的补充材料。
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