The Department of Pharmacology of Materia Medica (the State Key Laboratory of Functions and Applications of Medicinal Plants, The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, The High Educational Key Laboratory of Guizhou Province for Natural Medicianl Pharmacology and Drug Ability, The Union Key Laboratory of Guiyang City-Guizhou Medical Univeristy, The Key Laboratory of Optimal Utilizaiton of Natural Medicine Resources), School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guizhou, 550025, China; Department of Pharmacy, Guizhou Provincial People's Hospital, Guiyang City, Guizhou Province, China.
The Department of Pharmacology of Materia Medica (the State Key Laboratory of Functions and Applications of Medicinal Plants, The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, The High Educational Key Laboratory of Guizhou Province for Natural Medicianl Pharmacology and Drug Ability, The Union Key Laboratory of Guiyang City-Guizhou Medical Univeristy, The Key Laboratory of Optimal Utilizaiton of Natural Medicine Resources), School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guizhou, 550025, China; Department of Neonatal Disease Screening, Shenyang Maternity and Child Health Hospital, Shenyang City, Liaoning Province, China.
J Ethnopharmacol. 2019 Jun 12;237:149-158. doi: 10.1016/j.jep.2019.03.011. Epub 2019 Mar 14.
Fructus Alpinia zerumbet (FAZ), a dry and ripe fruit of Alpinia zerumbet (Pers.) Burtt. et Smith, is widely used as a spice to treat cardiovascular diseases in clinic as a miao folk medicine in Guizhou Province of China. Essential oil extracted from FAZ (EOFAZ) is the key bioactive ingredients.
This study aimed to examine the effects and mechanisms of EOFAZ on lipopolysaccharide (LPS)-induced endothelial cell injury, inflammation and apoptosis in vitro and in vivo.
For the in vitro study, LPS-treated human aortic endothelial cells were used to perform PCR, western blot analysis and immunofluorescence. For the in vivo study, male mouse were divided into four groups, vehicle control group and LPS group received 0.5% Tween-80 in saline; and two EOFAZ groups receive different dose of EOFAZ (90 mg kg ·day, 180 mg kg ·day) respectively. Each group was fed for 7 days by intragastrical administration at daily base. Then, except vehicle control group received saline, mice in other three groups were administered with LPS (1 mg kg , dissolved in saline) by intraperitoneal injection. 24 h later, Aorta tissue was collected and frozen immediately in liquid N, stored at -80 °C for western blot analysis.
We found that EOFAZ completely prevented LPS-induced HAEC activation and inflammation in vitro and in vivo, as assessed by expression of endothelial adhesion molecules, ICAM-1 and VCAM-1. Similarly, EOFAZ significantly blunted LPS-induced endothelial injury, as tested by MTT assay, LDH release and caspase-3 activation. We further demonstrated that TLR4-dependent NF-κB signaling may be involved in the process.
EOFAZ protected against LPS-induced endothelial cell injury and inflammation likely via inhibition of TLR4-dependent NF-κB signaling.
益智果实（FAZ），是姜科益智属植物益智（Alpinia oxyphylla Miq.）的干燥成熟果实，在中国贵州省作为一种民间药物，被广泛用作治疗心血管疾病的香料。从 FAZ 中提取的精油（EOFAZ）是关键的生物活性成分。
本研究旨在探讨 EOFAZ 对体外和体内脂多糖（LPS）诱导的内皮细胞损伤、炎症和凋亡的作用及其机制。
在体外研究中，使用 LPS 处理的人主动脉内皮细胞进行 PCR、western blot 分析和免疫荧光实验。在体内研究中，雄性小鼠分为四组，对照组给予生理盐水；LPS 组给予生理盐水；两个 EOFAZ 组分别给予不同剂量的 EOFAZ（90mg/kg·天，180mg/kg·天）。各组每天灌胃给药，连续 7 天。除对照组给予生理盐水外，其余三组均腹腔注射 LPS（1mg/kg，溶于生理盐水）。24 小时后，收集主动脉组织，立即用液氮冷冻，-80°C 储存，用于 western blot 分析。
我们发现，EOFAZ 完全阻止了 LPS 诱导的 HAEC 体外和体内激活和炎症，通过内皮细胞黏附分子 ICAM-1 和 VCAM-1 的表达来评估。同样，EOFAZ 显著减轻了 LPS 诱导的内皮损伤，通过 MTT 测定、LDH 释放和 caspase-3 激活来测试。我们进一步表明，TLR4 依赖性 NF-κB 信号通路可能参与了这一过程。
EOFAZ 通过抑制 TLR4 依赖性 NF-κB 信号通路，对 LPS 诱导的内皮细胞损伤和炎症具有保护作用。
