Xiao Rui-Yao, Wu Ling-Jing, Hong Xiao-Xiao, Tao Ling, Luo Peng, Shen Xiang-Chun
The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability, School of Pharmaceutical Science, Guizhou Medical University, Huaxi University Town, Guian New District, 550025, Guizhou, China.
Department of Pharmacognosy, School of Pharmaceutical Science, Guizhou Medical University, Huaxi University Town, Guian New District, 550025, Guizhou, China.
Biomed Chromatogr. 2018 Mar;32(3). doi: 10.1002/bmc.4112. Epub 2017 Nov 22.
Fructus Alpiniae zerumbet is widely used in Guizhou province as a miao folk herb with anti-inflammatory, analgesic, protection against cardiovascular diseases, antihypertension and antioxidant activities. To further investigate the chemical material basis, the spectrum-effect relationship was established using gray relational analysis between the chromatographic fingerprint and its bioactivities. Herein, the fingerprints of essential oils from Fructus Alpiniae zerumbet (EOFAZ) from various sources were determined by gas chromatography mass spectrometry, and the analgesic and anti-inflammatory bioactivities were investigated using the mouse model of acetic acid-induced writhing test and dimethylbenzene-induced mouse ear edema test. Finally, 17 common peaks were identified from nine batches of A. zerumbet, by comparison with the standard mass spectra in Nist2005, Wiley275 library. Meanwhile, the results showed significant analgesic and anti-inflammatory effects in all of the different sources of EOFAZ. In particularly, peak 1 (α-pipene), peak 3 (β-pinene), peak 9 (camphor) and peak 16 (α-cadinol) might be the main bioactive ingredients for analgesic and anti-inflammatory activities. The model of the spectrum-effect relationships of EOFAZ was successfully discovered, which provided a novel platform for finding the bioactive components, a theoretical foundation for its further study and helping to establish quality control of Fructus A. zerumbet.
艳山姜果实作为一种具有抗炎、镇痛、心血管疾病防护、抗高血压和抗氧化活性的苗族民间草药，在贵州省被广泛使用。为了进一步研究其化学物质基础，利用色谱指纹图谱与其生物活性之间的灰色关联分析建立了谱效关系。在此，采用气相色谱 - 质谱联用仪测定了不同来源的艳山姜果实挥发油指纹图谱（EOFAZ），并通过醋酸诱导扭体试验和二甲苯诱导小鼠耳肿胀试验的小鼠模型研究了其镇痛和抗炎生物活性。最后，通过与Nist2005、Wiley275库中的标准质谱图比较，从九批艳山姜中鉴定出17个共有峰。同时，结果表明所有不同来源的EOFAZ均具有显著的镇痛和抗炎作用。特别是，峰1（α-蒎烯）、峰3（β-蒎烯）、峰9（樟脑）和峰16（α-杜松醇）可能是镇痛和抗炎活性的主要生物活性成分。成功建立了EOFAZ的谱效关系模型，为寻找生物活性成分提供了一个新平台，为其进一步研究提供了理论基础，并有助于建立艳山姜果实的质量控制。
