Coates S R, Hansen D, Schecter G, Slutkin G, Hopewell P, Affronti L, Echenberg D F
J Clin Microbiol. 1986 Jul;24(1):126-30. doi: 10.1128/jcm.24.1.126-130.1986.
Seibert fractions prepared from Mycobacterium tuberculosis culture filtrates were evaluated by immunoblotting with a serum pool from patients with active pulmonary tuberculosis. Antibody activity was observed primarily with antigens in the polysaccharide II and A protein fractions; these fractions were further evaluated by immunoblotting with sera from individual patients with tuberculosis, from individuals without tuberculosis and positive for the purified protein derivative antigen skin test, and from individuals negative for the purified protein derivative antigen skin test. The antigens identified in the protein A fraction, a 32,000-molecular-weight antigen and a heterogeneous high-molecular-weight antigen, reacted with antibody found in sera from all patients with tuberculosis and with antibody from over 25% of the control individuals. A 10,000-molecular-weight antigen, a 30,000- to 44,000-molecular-weight antigen, and a heterogeneous high-molecular-weight antigen were observed in the polysaccharide II fraction; these antigens reacted with serum antibody from 70% or more of the patients with tuberculosis and with antibody from 20 to 70% of the control individuals. One of the antigens, with a molecular weight ranging from 17,000 to 28,000 in the polysaccharide II fraction, reacted with antibody in 64% of the sera from patients with tuberculosis but with only 1 of 15 control normal sera. This antigen may elicit an antibody response specifically associated with tuberculosis.
用活动性肺结核患者的混合血清通过免疫印迹法对从结核分枝杆菌培养滤液中制备的赛伯特级分进行评估。主要观察到抗体活性针对多糖II和A蛋白级分中的抗原；通过用来自患有结核病的个体、结核菌素纯蛋白衍生物抗原皮肤试验呈阳性的无结核病个体以及结核菌素纯蛋白衍生物抗原皮肤试验呈阴性的个体的血清进行免疫印迹法，对这些级分进行了进一步评估。在A蛋白级分中鉴定出的抗原，一种分子量为32,000的抗原和一种异质性高分子量抗原，与所有结核病患者血清中的抗体以及超过25%的对照个体血清中的抗体发生反应。在多糖II级分中观察到一种分子量为10,000的抗原、一种分子量为30,000至44,000的抗原以及一种异质性高分子量抗原；这些抗原与70%或更多结核病患者血清中的抗体以及20%至70%对照个体血清中的抗体发生反应。多糖II级分中分子量在17,000至28,000之间的一种抗原与64%的结核病患者血清中的抗体发生反应，但仅与15份对照正常血清中的1份发生反应。这种抗原可能引发与结核病特异性相关的抗体反应。
