Stroebel A B, Daniel T M, Lau J H, Leong J C, Richardson H
J Infect Dis. 1982 Aug;146(2):280-3. doi: 10.1093/infdis/146.2.280.
Sera from patients receiving treatment for active bone and joint tuberculosis and sera from patients with inactive bone and joint tuberculosis were examined by an enzyme-linked immunosorbent assay for antibody to antigen 6, a homogeneous protein prepared from Mycobacterium tuberculosis strain H37Ra by immunosorbent affinity chromatography. Sera from 21 control subjects had a geometric mean titer of 1:6 with no difference between tuberculin purified protein derivative-positive and -negative patients. Sera from 20 patients with inactive disease had a geometric mean titer of 1:19. Fifteen patients receiving treatment for M. tuberculosis infection had a geometric mean titer of 1:179, which is significantly different from the geometric mean titers of both of the patients with inactive tuberculosis (P less than 0.001) and the control subjects (P less than 0.001). At a cut-off titer of 1:32, the sensitivity of the assay is 94% and the specificity for the control subjects and patients with inactive disease was 100%.
采用酶联免疫吸附试验检测正在接受活动性骨与关节结核治疗患者的血清以及非活动性骨与关节结核患者的血清，以检测其针对抗原6的抗体。抗原6是一种通过免疫吸附亲和层析从结核分枝杆菌H37Ra菌株制备的纯蛋白。21名对照受试者的血清几何平均滴度为1:6，结核菌素纯蛋白衍生物阳性和阴性患者之间无差异。20名非活动性疾病患者的血清几何平均滴度为1:19。15名正在接受结核分枝杆菌感染治疗的患者的血清几何平均滴度为1:179，这与非活动性结核患者（P<0.001）和对照受试者（P<0.001）的几何平均滴度均有显著差异。在滴度截断值为1:32时，该检测方法的敏感性为94%，对对照受试者和非活动性疾病患者的特异性为100%。
