采用放射免疫分析法和酶联免疫吸附测定法检测结核病患者针对纯化蛋白衍生物的免疫球蛋白G抗体。
Detection of immunoglobulin G antibody to purified protein derivative in patients with tuberculosis by radioimmunoassay and enzyme-linked immunosorbent assay.
作者信息
Zeiss C R, Radin R C, Williams J E, Levitz D, Phair J P
出版信息
J Clin Microbiol. 1982 Jan;15(1):93-6. doi: 10.1128/jcm.15.1.93-96.1982.
Sera from patients with active tuberculosis and sera from appropriate control individuals were assayed for immunoglobulin G antibody activity to purified protein derivative by a polystyrene tube radioimmunoassay and an enzyme-linked immunosorbent assay. Both assays showed a marked increase in immunoglobulin G antibody activity in patients with active tuberculosis. There was no overlap between the values for the patient group and the values for the purified protein derivative skin test-positive control individuals. The replication of these assays was excellent, and both could provide quantitative measurements of immunoglobulin G antibody activity to purified protein derivative antigen within 24 h. These techniques have potential as rapid diagnostic aids in evaluating patients with suspected active tuberculosis.
采用聚苯乙烯管放射免疫分析法和酶联免疫吸附测定法，对活动性肺结核患者的血清以及相应对照个体的血清进行检测，以测定其针对纯化蛋白衍生物的免疫球蛋白G抗体活性。两种检测方法均显示，活动性肺结核患者的免疫球蛋白G抗体活性显著增加。患者组的值与纯化蛋白衍生物皮肤试验阳性对照个体的值之间没有重叠。这些检测方法的重复性极佳，且两者均能在24小时内对针对纯化蛋白衍生物抗原的免疫球蛋白G抗体活性进行定量测定。这些技术在评估疑似活动性肺结核患者时，有作为快速诊断辅助手段的潜力。
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