Kalish S B, Radin R C, Phair J P, Levitz D, Zeiss C R, Metzger E
J Infect Dis. 1983 Mar;147(3):523-30. doi: 10.1093/infdis/147.3.523.
Sera from patients with active pulmonary tuberculosis and pulmonary diseases frequently mimicking tuberculosis were assayed for immunoglobulin G antibody activity to purified protein derivative (PPD) by an enzyme-linked immunosorbent assay. A method of standardization was developed to limit assay variation. Patients with active pulmonary tuberculosis had a significantly greater mean level of antibody than had patients with atypical tuberculosis (P = 0.005), sarcoidosis (P = 0.0001), histoplasmosis (P = 0.004), blastomycosis (P = 0.008), or cryptococcosis (P = 0.017), patients who had received bacille Calmette-Guérin vaccination (P = 0.003) or who had a history of treated tuberculosis (P = 0.003), and PPD skin test-positive and skin test-negative control subjects (P = 0.001). This technique may have potential use as a rapid diagnostic aid in evaluating patients with suspected active pulmonary tuberculosis.
采用酶联免疫吸附试验，对活动性肺结核患者以及常被误诊为肺结核的肺部疾病患者的血清进行检测，以测定其针对纯化蛋白衍生物（PPD）的免疫球蛋白G抗体活性。我们开发了一种标准化方法，以减少检测变异。活动性肺结核患者的平均抗体水平显著高于非典型肺结核患者（P = 0.005）、结节病患者（P = 0.0001）、组织胞浆菌病患者（P = 0.004）、芽生菌病患者（P = 0.008）、隐球菌病患者（P = 0.017）、接受过卡介苗接种的患者（P = 0.003）、有肺结核治疗史的患者（P = 0.003）以及PPD皮肤试验阳性和阴性对照受试者（P = 0.001）。这项技术可能在评估疑似活动性肺结核患者时作为一种快速诊断辅助手段具有潜在用途。
