Allan B J, White T T, Reber H A, Roberts C, Schilling J J
Dig Dis Sci. 1982 May;27(5):437-43. doi: 10.1007/BF01295653.
Pancreatic secretions from the hamster model for pancreatic ductal adenocarcinoma were analyzed to determine whether alterations had occurred in the protein composition. CCK-and secretin-stimulated secretions were collected from 13 animals with cancer and 16 normal controls. Subsequent separation of the proteins by isoelectric focusing showed the following: (1) Significant changes occurred in the protein composition from animals with carcinoma. An unusually dark band was present at pH 7.5 just below amylase (pH 7.65); two unidentified bands, present in the normals at pH 6.9 and 7.0, were missing; and marked decreases occurred in the cathodic proteins with isoelectric points above pH 9, (2) CCK provided the optimal stimulus for differentiating specimens from animals with carcinoma from the normal controls. (3) The protein concentrations of CCK-stimulated secretions of animals with carcinoma were significantly lower than the controls. We have concluded that the protein alterations which have occurred in the hamster model for pancreatic ductal adenocarcinoma warrant further investigation.
对仓鼠胰腺导管腺癌模型的胰腺分泌物进行分析，以确定蛋白质组成是否发生了改变。从13只患癌动物和16只正常对照动物中收集胆囊收缩素（CCK）和促胰液素刺激后的分泌物。随后通过等电聚焦对蛋白质进行分离，结果如下：（1）患癌动物的蛋白质组成发生了显著变化。在pH 7.5处，紧挨着淀粉酶（pH 7.65）下方出现了一条异常深的条带；在正常动物中pH 6.9和7.0处存在的两条未鉴定条带缺失；并且等电点高于pH 9的阴极蛋白质显著减少。（2）CCK为区分患癌动物与正常对照动物的标本提供了最佳刺激。（3）患癌动物CCK刺激分泌物的蛋白质浓度显著低于对照动物。我们得出结论，仓鼠胰腺导管腺癌模型中发生的蛋白质改变值得进一步研究。
