White T T, Allan B J, Schilling J J, Miyashita H
Dig Dis Sci. 1983 Sep;28(9):792-800. doi: 10.1007/BF01296901.
Pancreatic cancer secretory protein profiles were shown to be different from normals by a microcomputer-assisted analysis of isoelectric focusing patterns. Two acidic protein band regions (pI 3.0 and 4.5) of the pancreatic carcinoma profiles were significantly increased, and eight protein bands (pI 3.7, 5.0, 5.5, 6.5, 6.9, 7.3, 8.0, greater than 10.0) were significantly decreased. Highly significant decreases occurred at pH 7.3 (chymotrypsinogen) and at pH 5.0 (procarboxypeptidase Al, DNase I) in nonactivated specimens. The ratio between the absorbance points at 2.08 and 2.63 cm from the anode in each protein pattern differentiated the pancreas cancer specimens from the control group. Profiles found for the control group gave pI values similar to those found in the literature. The potential value of these findings in the search for tumor markers warrants further investigation as to whether these specimens can be differentiated from those from chronic pancreatitis patients.
通过对等电聚焦图谱进行微机辅助分析，发现胰腺癌分泌蛋白谱与正常情况不同。胰腺癌图谱中的两个酸性蛋白条带区域（pI 3.0和4.5）显著增加，而八个蛋白条带（pI 3.7、5.0、5.5、6.5、6.9、7.3、8.0、大于10.0）显著减少。在未激活的标本中，pH 7.3（胰凝乳蛋白酶原）和pH 5.0（羧肽酶原A1、脱氧核糖核酸酶I）处出现高度显著的减少。每种蛋白质图谱中距阳极2.08和2.63厘米处吸光度点的比值可区分胰腺癌标本与对照组。对照组的图谱给出的pI值与文献中发现的值相似。这些发现对于寻找肿瘤标志物的潜在价值值得进一步研究，以确定这些标本是否能与慢性胰腺炎患者的标本区分开来。
