Moraes O S, Cardoso B F, Pacheco T A, Pinto A Z L, Carvalho M S, Hahn R C, Burlamaqui T C T, Oliveira L F, Oliveira R S, Vasconcelos J M, Lemos P S, Nunes M R T, Slhessarenko R D
Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Brazil.
Centro de Inovação Tecnológica, Instituto Evandro Chagas, Ministério da Saúde, Ananindeua, Brazil.
Med Vet Entomol. 2019 Sep;33(3):397-406. doi: 10.1111/mve.12374. Epub 2019 Mar 19.
New species of insect-specific viruses (ISV) have been reported worldwide. In the present study, the complete genome of Culex flavivirus (CxFV) and partial sequences of other ISVs in Culex quinquefasciatus Say 1823 females (n = 3425) sampled in 200 urban areas census tracts of Cuiaba, state of Mato Grosso, were identified via reverse transcriptase-polymerase chain reaction for a NS5 region of flaviviruses, nucleotide and high-throughput sequencing, and viral isolation in C6/36 cells. CxFV was detected in 16 of 403 mosquito pools; sequences found in the study presented a high similarity with isolates from São Paulo, Brazil and other countries in Latin American that belong to genotype II, supporting the geographical influence on CxFV evolution. The monthly maximum likelihood estimation for CxFV ranged from 1.81 to 9.94 per 1000 mosquitoes. In addition to the CxFV complete genome, one pool contained an ORF1 sequence (756 bp) that belongs to a novel Negevirus from the Sandewavirus supergroup most similar to the Santana virus (77.1%) and another pool presented an RNA-dependent RNA polymerase sequence (1081 bp) of a novel Rhabdovirus most similar to Wuhan mosquito virus 9 (44%). After three passages in C6/36 cells, only CxFV was isolated from these co-infected pools. The importance of ISVs relies on their possible ability to interfere with arbovirus replication in competent vectors.
全球已报道了多种昆虫特异性病毒（ISV）。在本研究中，通过逆转录聚合酶链反应针对黄病毒的NS5区域、核苷酸和高通量测序以及在C6/36细胞中的病毒分离，鉴定了在巴西马托格罗索州库亚巴市200个城市普查区域采集的3425只致倦库蚊（Culex quinquefasciatus Say 1823）雌蚊中库蚊黄病毒（CxFV）的完整基因组以及其他ISV的部分序列。在403个蚊群中的16个中检测到了CxFV；研究中发现的序列与来自巴西圣保罗和拉丁美洲其他国家的属于基因型II的分离株高度相似，这支持了地理因素对CxFV进化的影响。CxFV的每月最大似然估计范围为每1000只蚊子1.81至9.94。除了CxFV完整基因组外，一个蚊群包含一个属于Sandewavirus超群的新型内盖夫病毒的ORF1序列（756 bp），与桑塔纳病毒最相似（77.1%），另一个蚊群呈现出一种与武汉蚊病毒9最相似的新型弹状病毒的RNA依赖性RNA聚合酶序列（1081 bp）（44%）。在C（6/36）细胞中传代三次后，仅从这些共感染的蚊群中分离出了CxFV。ISV的重要性在于它们可能具有干扰虫媒病毒在易感媒介中复制的能力。
