College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China.
Ecotoxicol Environ Saf. 2019 Jul 15;175:74-82. doi: 10.1016/j.ecoenv.2019.03.036. Epub 2019 Mar 16.
Lead (Pb) is a type of toxic metal that can hurt the immune system. Selenium (Se) can reduce the damage caused by heavy metals. To investigate the effects of Se against Pb on bird immune cells, as well as the immunotoxin mechanism of Pb, Se supplementation and/or Pb poisoning chicken models were established. One hundred eighty 1-year-old broiler chickens were randomly divided into four groups (n = 6). The four groups were the control group, the selenium-rich group (Se group), the Pb supplementation group (Pb group) and the Se and Pb compound group (Se + Pb group). The peripheral blood lymphocytes of chickens were collected to test the selenoproteins and cytokine mRNA levels at 30 and 60 d. Determination of the content of Se and Pb in the serum, principal component analysis and ingenuity pathway analysis were performed at the two time points. As a result, Pb exposure increased the content of Pb, activating the Th1/Th2 pathway in peripheral blood lymphocytes. Additionally, this experiment showed that Se supplementation and Pb exposure could influence the mRNA levels of selenoproteins and cytokines in the peripheral blood lymphocytes of chickens. However, all of the parameters that we detected in the experiment indicated that Se supplementation could alleviate the increase of selenoproteins and cytokine mRNA levels and the Th1/Th2 imbalance induced by Pb in peripheral blood lymphocytes. In summary, Se can alleviate the toxic effects caused by Pb in the peripheral blood lymphocytes of chickens, suggesting the antagonism between Se and Pb.
铅（Pb）是一种有毒金属，会损害免疫系统。硒（Se）可以减轻重金属造成的损害。为了研究硒（Se）对铅（Pb）对鸟类免疫细胞的影响，以及铅（Pb）的免疫毒素机制，建立了硒（Se）补充和/或铅（Pb）中毒鸡模型。将 180 只 1 岁的肉鸡随机分为四组（n = 6）。这四组分别为对照组、富硒组（Se 组）、铅（Pb）补充组（Pb 组）和硒（Se）和铅（Pb）复合组（Se + Pb 组）。采集鸡外周血淋巴细胞，分别于 30 和 60 d 检测硒蛋白和细胞因子 mRNA 水平。在这两个时间点，测定血清中 Se 和 Pb 的含量，进行主成分分析和 ingenuity 通路分析。结果表明，Pb 暴露增加了 Pb 的含量，激活了外周血淋巴细胞中的 Th1/Th2 通路。此外，本实验表明，硒（Se）补充和 Pb 暴露可以影响鸡外周血淋巴细胞中硒蛋白和细胞因子的 mRNA 水平。然而，我们在实验中检测到的所有参数都表明，硒（Se）补充可以减轻 Pb 在外周血淋巴细胞中引起的硒蛋白和细胞因子 mRNA 水平升高以及 Th1/Th2 失衡。综上所述，硒（Se）可以减轻鸡外周血淋巴细胞中铅（Pb）的毒性作用，提示硒（Se）与铅（Pb）之间存在拮抗作用。
