Department of Science, Roma Tre University, Via della Vasca navale 79, Roma, Italy.
Department of Engineering, Roma Tre University, via Vito Volterra 62, Roma, Italy.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1133-1140. doi: 10.1016/j.msec.2019.02.052. Epub 2019 Feb 15.
In this work, a new covalent grafting of chitosan on titanium and Ti6Al4V alloy surfaces is reported using chloroacetic acid as linker agent. Good results were obtained both on titanium and on Ti6Al4V alloy. The effect of the surface acid pretreatments on the subsequent functionalization with chitosan is evaluated. The morphological aspect of acid etched metal surfaces before chitosan grafting has been characterized by scanning electron microscopy (SEM). The presence of carboxylic groups on metal surfaces and then the efficiency of chitosan covalent immobilization were detected by Fourier transformed infrared-Attenuated Total Reflectance (FTIR-ATR) and X-Ray photoelectron spectroscopy (XPS). Cyclic voltammetry tests, using the functionalized titanium and Ti6Al4V samples as electrodes, were conducted in different aqueous solutions, to detect the presence of the homogeneous overlayer of chitosan on the surface, and to evaluate the importance of the carboxyl groups as linker agent.
在这项工作中，使用氯乙酸作为连接剂，报道了壳聚糖在钛和 Ti6Al4V 合金表面的新共价接枝。在钛和 Ti6Al4V 合金上均获得了良好的结果。评估了表面酸预处理对随后用壳聚糖功能化的影响。通过扫描电子显微镜（SEM）对壳聚糖接枝前酸蚀金属表面的形态进行了表征。通过傅里叶变换衰减全反射红外光谱（FTIR-ATR）和 X 射线光电子能谱（XPS）检测金属表面上羧酸基团的存在以及壳聚糖共价固定化的效率。使用功能化的钛和 Ti6Al4V 样品作为电极，在不同的水溶液中进行循环伏安测试，以检测壳聚糖在表面上的均匀覆盖层的存在，并评估羧基作为连接剂的重要性。
