Materials Science and Engineering, Gebze Technical University, 41400, Gebze, Turkey.
Industrial Engineering, Giresun University, 28200, Giresun, Turkey.
Sci Rep. 2021 Jul 23;11(1):15104. doi: 10.1038/s41598-021-94502-z.
Ca-based porous and rough bioceramic surfaces were coated onto zirconium by micro-arc oxidation (MAO). Subsequently, the MAO-coated zirconium surfaces were covered with an antimicrobial chitosan layer via the dip coating method to develop an antimicrobial, bioactive, and biocompatible composite biopolymer and bioceramic layer for implant applications. Cubic ZrO, metastable CaZrO, and Ca(PO) were detected on the MAO surface by powder-XRD. The existence of chitosan on the MAO-coated Zr surfaces was verified by FTIR. The micropores and thermal cracks on the bioceramic MAO surface were sealed using a chitosan coating, where the MAO surface was porous and rough. All elements such as Zr, O, Ca, P, and C were homogenously distributed across both surfaces. Moreover, both surfaces indicated hydrophobic properties. However, the contact angle of the MAO surface was lower than that of the chitosan-based MAO surface. In vitro bioactivity on both surfaces was investigated via XRD, SEM, and EDX analyses post-immersion in simulated body fluid (SBF) for 14 days. In vitro bioactivity was significantly enhanced on the chitosan-based MAO surface with respect to the MAO surface. In vitro microbial adhesions on the chitosan-based MAO surfaces were lower than the MAO surfaces for Staphylococcus aureus and Escherichia coli.
钙基多孔粗糙生物陶瓷表面通过微弧氧化（MAO）涂覆到锆上。随后，通过浸涂法在 MAO 涂层锆表面覆盖抗菌壳聚糖层，以开发用于植入物应用的抗菌、生物活性和生物相容的复合生物聚合物和生物陶瓷层。粉末 XRD 检测到 MAO 表面上存在立方 ZrO、亚稳 CaZrO 和 Ca(PO)。FTIR 验证了 MAO 涂层 Zr 表面上壳聚糖的存在。壳聚糖涂层封闭了生物陶瓷 MAO 表面上的微孔和热裂纹，MAO 表面多孔且粗糙。Zr、O、Ca、P 和 C 等所有元素在两个表面上均匀分布。此外，两个表面都表现出疏水性。然而，MAO 表面的接触角低于基于壳聚糖的 MAO 表面。在模拟体液（SBF）中浸泡 14 天后，通过 XRD、SEM 和 EDX 分析研究了两个表面的体外生物活性。与 MAO 表面相比，基于壳聚糖的 MAO 表面的体外生物活性显著提高。壳聚糖基 MAO 表面上金黄色葡萄球菌和大肠杆菌的体外微生物黏附性低于 MAO 表面。
