College of Materials Science and Engineering, Nanjing Tech University, Nanjing 210009, China.
Department of Clinical Laboratory, People's Hospital of Jinhu Country, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:979-985. doi: 10.1016/j.msec.2019.02.049. Epub 2019 Feb 15.
Amino-grafted and vaterite-contained tricalcium silicate cement (A-V-CS) was composited with 58S bioglass/chitosan/carboxy methyl cellulose (BG/CS/CMC, referred as BGs) to surmount the non-absorbability and infection problems of traditional bone wax. Its material, bioactive, biocompatible and antibacterial properties were systematically characterized. The results revealed that A-V-CS/BGs possessed self-setting, injectable, mechanical and degradable abilities. A-V-CS/BGs (1:1 g/g) was optimum owing to its higher compressive strength (9.91 MPa) and lower pH value (7.6 to 8.1) in the test groups. In vitro immersion experiment demonstrated that A-V-CS/BGs had good hydroxyapatite formation ability, and its excellent cell adhesion, low cytotoxicity and superior cell proliferation were verified by mouse embryonic osteoblast precursor cells in cell tests. Compared with A-V-CS, antibacterial experiment illustrated the significantly enhanced antibacterial property of A-V-CS/BGs to Staphylococcus aureus and Escherichia coli.
氨基接枝和含文石碳酸钙的硅酸三钙水泥（A-V-CS）与 58S 生物玻璃/壳聚糖/羧甲基纤维素（BG/CS/CMC，简称 BGs）复合，以克服传统骨蜡的不可吸收性和感染问题。对其材料、生物活性、生物相容性和抗菌性能进行了系统的表征。结果表明，A-V-CS/BGs 具有自凝固、可注射、机械和可降解能力。在实验组中，A-V-CS/BGs（1:1g/g）因其较高的抗压强度（9.91MPa）和较低的 pH 值（7.6 至 8.1）而达到最佳效果。体外浸泡实验表明，A-V-CS/BGs 具有良好的羟基磷灰石形成能力，细胞实验通过小鼠胚胎成骨前体细胞验证了其良好的细胞黏附性、低细胞毒性和优异的细胞增殖能力。与 A-V-CS 相比，抗菌实验表明 A-V-CS/BGs 对金黄色葡萄球菌和大肠杆菌的抗菌性能有显著提高。
