Bechtner Julia, Xu Di, Behr Jürgen, Ludwig Christina, Vogel Rudi F
Lehrstuhl für Technische Mikrobiologie, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
Bavarian Center for Biomolecular Mass Spectrometry, Freising, Germany.
Front Microbiol. 2019 Feb 28;10:325. doi: 10.3389/fmicb.2019.00325. eCollection 2019.
Water kefir is a slightly alcoholic and traditionally fermented beverage, which is prepared from sucrose, water, kefir grains, and dried or fresh fruits (e.g., figs). () , and () are predominant and stable lactic acid bacteria and yeasts, respectively, isolated from water kefir consortia. The growth of and are improved in the presence of . In this work we demonstrate that quantitative comparative proteomics enables the investigation of interactions between LAB and yeast to predict real-time metabolic exchange in water kefir. It revealed 73 differentially expressed (DE) in TMW 1.1827 in the presence of . The presence of the yeast induced changes in the changes in the carbohydrate metabolism of and affected reactions involved in NAD/NADH homeostasis. Furthermore, the DE enzymes involved in amino acid biosynthesis or catabolism predict that releases glutamine, histidine, methionine, and arginine, which are subsequently used by to ensure its survival in the water kefir consortium. In co-culture with profits from riboflavin, most likely secreted by the yeast. The reaction of to the presence of differs from that one of the previously studied , which displays 233 differentially expressed proteins, changes in citrate metabolism and an antidromic strategy for NAD/NADH homeostasis. So far, aggregation promotion factors, i.e., formation of a specific glucan and bifunctional enzymes were only detected in .
水开菲尔是一种略带酒精味的传统发酵饮料，由蔗糖、水、开菲尔粒和干制或新鲜水果（如无花果）制成。从水开菲尔菌群中分离出的（）和（）分别是占主导地位且稳定的乳酸菌和酵母菌。在（）存在的情况下，（）和（）的生长得到改善。在这项工作中，我们证明定量比较蛋白质组学能够研究乳酸菌和酵母菌之间的相互作用，以预测水开菲尔中的实时代谢交换。研究发现，在（）存在的情况下，TMW 1.1827中有73种差异表达蛋白。酵母菌的存在导致（）的碳水化合物代谢发生变化，并影响了与NAD/NADH稳态相关的反应。此外，参与氨基酸生物合成或分解代谢的差异表达酶预测，（）会释放谷氨酰胺、组氨酸、蛋氨酸和精氨酸，随后（）会利用这些氨基酸来确保其在水开菲尔菌群中的存活。与（）共培养时，（）受益于可能由酵母菌分泌的核黄素。（）对（）存在的反应与之前研究的（）不同，（）显示出233种差异表达蛋白、柠檬酸代谢变化以及NAD/NADH稳态的反向策略。到目前为止，仅在（）中检测到聚集促进因子，即特定葡聚糖的形成和双功能酶。
