Xia Zhengchao, Zhou Xuelin, Li Jingyi, Li Lei, Ma Yi, Wu Yi, Huang Zhong, Li Xiaorong, Xu Pingxiang, Xue Ming
Department of Pharmacology, Beijing Laboratory for Biomedical Detection Technology and Instrument, School of Basic Medical Sciences, Capital Medical University, Beijing, China.
Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Front Microbiol. 2019 Jun 27;10:1398. doi: 10.3389/fmicb.2019.01398. eCollection 2019.
Although the biological processes of organism under hypoxic stress had been elucidated, the whole physiological changes of are still unclear. In this work, we investigated the changes of biological process of under hypoxia by the methods of transcriptomics, proteomics, metabolomics, and bioinformatics. The results showed that the expression of a total of 1017 mRNA in transcriptome, 213 proteins in proteome, and 51 metabolites in metabolome had been significantly changed between the hypoxia and normoxia conditions. Moreover, based on the integration of system-omics data, we found that the carbohydrate, amino acids, fatty acid biosynthesis, lipid metabolic pathway, and oxidative phosphorylation were significantly changed in hypoxic stress. Among these pathways, the glycerophospholipid metabolic pathway was remarkably up-regulated from the mRNA, protein, and metabolites levels under hypoxic stress, and the expression of relevant mRNA was also confirmed by the qPCR. The metabolites of glycerophospholipid pathway such as phosphatidylcholine, phosphatidylethanolamine, phosphoinositide, and phosphatidic acids probably maintained the stability of cell membranes against hypoxic stress to relieve the cell injury, and kept survive with energy production. These findings in the hypoxic omics and integrated networks provide very useful information for further exploring the molecular mechanism of hypoxic stress.
尽管缺氧应激下生物体的生物学过程已得到阐明，但其整体生理变化仍不清楚。在这项工作中，我们通过转录组学、蛋白质组学、代谢组学和生物信息学方法研究了缺氧条件下生物体的生物学过程变化。结果表明，在缺氧和常氧条件之间，转录组中共有1017种mRNA、蛋白质组中213种蛋白质以及代谢组中51种代谢物的表达发生了显著变化。此外，基于多组学数据的整合，我们发现碳水化合物、氨基酸、脂肪酸生物合成、脂质代谢途径和氧化磷酸化在缺氧应激中发生了显著变化。在这些途径中，甘油磷脂代谢途径在缺氧应激下从mRNA、蛋白质和代谢物水平均显著上调，并且相关mRNA的表达也通过qPCR得到了证实。甘油磷脂途径的代谢物如磷脂酰胆碱、磷脂酰乙醇胺、磷酸肌醇和磷脂酸可能维持细胞膜对缺氧应激的稳定性以减轻细胞损伤，并通过能量产生维持生物体存活。这些缺氧组学和整合网络中的发现为进一步探索缺氧应激的分子机制提供了非常有用的信息。
