(L)叶提取物的挥发油植物化学筛选、抗菌活性及抗氧化性能
Phytochemical Screening of Essential Oils and Antibacterial Activity and Antioxidant Properties of (L) Leaf Extract.
作者信息
Umaru Isaac John, Badruddin Fasihuddin A, Umaru Hauwa A
机构信息
Faculty of Resource Science and Technology, University of Malaysia Sarawak, Kuching, Kota Samarahan, Malaysia.
出版信息
Biochem Res Int. 2019 Feb 12;2019:7143989. doi: 10.1155/2019/7143989. eCollection 2019.
OBJECTIVE
To ascertain the essential oil phytochemicals of the leaf and to test for the antibacterial and antioxidant properties of dichloromethane crude extract of leaf.
METHODS
The phytochemical screening of essential oils, extraction by hydrodistillation using the Clevenger apparatus, and analysis performed by gas chromatography equipped with a flame ionization detector (GC-FID). Antibacterial activity and the inhibition rate (mm) were determined using the agar disc method against four bacterial strains using tetracycline as positive control. The antioxidant potential of dichloromethane crude extract was investigated spectrophotometrically using 1,1-diphenyl-2-picrylhydrazyl.
RESULTS
The essential oil properties were reasonable with major phytochemicals like uncineol 30.9%, eicosane 27.4%, eicosane 21.6%, and 4-propyl-guaiacol 14.05%. The antibacterial activity of the dichloromethane crude extract showed broad-spectrum activity against , , , and with inhibition value ranges between 2.50 ± 0.10 mm and 5.00 ± 0.06 mm. The dichloromethane crude extract exhibited strong antioxidant activities when compared to the standard.
CONCLUSIONS
These results suggest that the leaves of is composed of essential compound as well as antibacterial and antioxidant properties from the crude extract; these are possible due to the presence of some bioactive compounds in the crude extract. The species also showed a reasonable amount of natural products in the essential oils from the hydrodistillation which can as well be used in the cosmetics and food industries.
目的
确定该植物叶片的精油植物化学成分，并测试叶片二氯甲烷粗提物的抗菌和抗氧化性能。
方法
对精油进行植物化学筛选，使用克莱文杰装置通过水蒸馏法提取，并通过配备火焰离子化检测器的气相色谱仪（GC-FID）进行分析。以四环素作为阳性对照，采用琼脂纸片法测定对四种细菌菌株的抗菌活性及抑菌率（毫米）。使用1,1-二苯基-2-苦基肼分光光度法研究二氯甲烷粗提物的抗氧化潜力。
结果
精油特性良好，主要植物化学成分如钩樟醇30.9%、二十烷27.4%、二十烷21.6%和4-丙基愈创木酚14.05%。二氯甲烷粗提物的抗菌活性对[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]表现出广谱活性，抑菌值范围在2.50±0.10毫米至5.00±0.06毫米之间。与标准品相比，二氯甲烷粗提物表现出较强的抗氧化活性。
结论
这些结果表明该植物的叶片由精油中的必需化合物以及粗提物的抗菌和抗氧化性能组成；这可能是由于粗提物中存在一些生物活性化合物。该物种在水蒸馏得到的精油中也含有相当数量的天然产物，可用于化妆品和食品工业。
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