Olukiran Olaoluwa Sesan, Akomolafe Rufus Ojo, Ilesanmi Olutosin Samuel, Imafidon Christian Eseigbe, Alabi Quadri Kunle
Department of Physiological Sciences Faculty of Basic Medical Sciences Obafemi Awolowo University Ile-Ife Nigeria.
Department of Biochemistry and Molecular Biology Faculty of Sciences Obafemi Awolowo University Ile-Ife Nigeria.
Animal Model Exp Med. 2018 Nov 14;1(4):295-304. doi: 10.1002/ame2.12035. eCollection 2018 Dec.
The study determined the fractions of proteins in the urine and plasma of rats at different ages, measured the plasma and urine concentrations of markers of renal function, with a view to determining the influence of proteinuria on renal function.
Eighty Wistar rats were used for this study. Groups 1 and 2 each consisted of eight 1-month-old male and female rats; 3 and 4 had eight 3-month-old male and female rats; 5 and 6 had eight 6-month-old male and female rats; 7 and 8 had eight 9-month old male and female rats; and 9 and 10 had eight 12-month-old male and female rats.
A fraction of the molecular weight of protein in the urine of rats aged 1, 9 and 12 months was higher than that of 3 and 6 months. The total protein concentration in the urine of male and female rats aged 9 and 12 months was significantly higher than that of rats aged 1 and 3 months. The urine creatinine concentrations of male and female rats aged 9 months were significantly higher when compared with that of 1, 3, 6 and 12 months.
Our results suggest that the 3-month-old rats seem less affected by proteinuria, because they had the least urine protein, and consistent and reduced plasma and urine concentrations of markers of renal function. The results of this study may provide a foundation for future mechanistic inquiries as to why this age group was the least affected by proteinuria.
本研究测定了不同年龄大鼠尿液和血浆中的蛋白质组分，测量了肾功能标志物的血浆和尿液浓度，旨在确定蛋白尿对肾功能的影响。
本研究使用了80只Wistar大鼠。第1组和第2组各有8只1月龄的雄性和雌性大鼠；第3组和第4组有8只3月龄的雄性和雌性大鼠；第5组和第6组有8只6月龄的雄性和雌性大鼠；第7组和第8组有8只9月龄的雄性和雌性大鼠；第9组和第10组有8只12月龄的雄性和雌性大鼠。
1月龄、9月龄和12月龄大鼠尿液中蛋白质的分子量组分高于3月龄和6月龄大鼠。9月龄和12月龄雄性和雌性大鼠尿液中的总蛋白浓度显著高于1月龄和3月龄大鼠。9月龄雄性和雌性大鼠的尿肌酐浓度与1月龄、3月龄、6月龄和12月龄大鼠相比显著更高。
我们的结果表明，3月龄大鼠受蛋白尿影响似乎较小，因为它们的尿蛋白最少，且肾功能标志物的血浆和尿液浓度持续降低。本研究结果可能为未来探究该年龄组受蛋白尿影响最小的原因提供机制研究基础。
