Pollard Amelia K, Ingram Thomas L, Ortori Catharine A, Shephard Freya, Brown Margaret, Liddell Susan, Barrett David A, Chakrabarti Lisa
School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Centre for Analytical Bioscience, School of Pharmacy University of Nottingham, NG7 2RD, UK.
Aging (Albany NY). 2019 Mar 19;11(6):1664-1685. doi: 10.18632/aging.101861.
It is accepted that smaller mammals with higher metabolic rates have shorter lifespans. The very few species that do not follow these rules can give insights into interesting differences. The recorded maximum lifespans of bats are exceptional - over 40 years, compared with the laboratory mouse of 4 years. We investigated the differences in the biochemical composition of mitochondria between bat and mouse species. We used proteomics and ultra-high-performance liquid chromatography coupled with high resolution mass spectrometry lipidomics, to interrogate mitochondrial fractions prepared from and brain and skeletal muscle. Fatty acid binding protein 3 was found at different levels in mouse and bat muscle mitochondria and its orthologues were investigated in knock-downs for LBP 4, 5 and 6. In the bat, high levels of free fatty acids and N-acylethanolamine lipid species together with a significantly greater abundance of fatty acid binding protein 3 in muscle (1.8-fold, =0.037) were found. Manipulation of fatty acid binding protein orthologues in suggest these proteins and their role in lipid regulation are important for mitochondrial function.
人们普遍认为，代谢率较高的小型哺乳动物寿命较短。极少数不遵循这些规律的物种可以为有趣的差异提供见解。蝙蝠的记录最大寿命非常特殊——超过40年，而实验室小鼠的寿命为4年。我们研究了蝙蝠和小鼠物种线粒体生化组成的差异。我们使用蛋白质组学和超高效液相色谱与高分辨率质谱脂质组学相结合的方法，对从大脑和骨骼肌中制备的线粒体组分进行分析。在小鼠和蝙蝠肌肉线粒体中发现了不同水平的脂肪酸结合蛋白3，并在LBP 4、5和6的敲低实验中对其同源物进行了研究。在蝙蝠中，发现肌肉中游离脂肪酸和N-酰基乙醇胺脂质种类的水平较高，同时脂肪酸结合蛋白3的丰度显著更高（1.8倍，P=0.037）。对脂肪酸结合蛋白同源物的操纵表明，这些蛋白质及其在脂质调节中的作用对线粒体功能很重要。
