Bedeloğlu Elçin, Ersanlı Selim, Arısan Volkan
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, İstanbul Aydın University, Istanbul, Turkey.
Department of Oral Implantology, Faculty of Dentistry, Capa, Istanbul University, Istanbul, Turkey.
J Dent Sci. 2017 Mar;12(1):7-13. doi: 10.1016/j.jds.2016.04.005. Epub 2016 Jul 9.
BACKGROUND/PURPOSE: The presence of adequate bone volume is a critical factor in rehabilitative dentistry. Despite the use of many promising alloplasts, success in stimulating bone formation has been limited, mostly due to poor local biological response. Growth factors have been introduced to stimulate angiogenesis and new bone formation. This histologic and histomorphometric study aimed to evaluate the effect of vascular endothelial growth factor (VEGF) and a biphasic alloplastic graft material (BA) on the healing of endosseous defects in rats.
Twenty male Wistar rats were used. Two critical-sized bone defects were created in both the right and left femurs of each rat. Each defect was randomly assigned to be treated with VEGF, BA, or VEGF + BA, or to be left empty as a control. Half of the animals were sacrificed after 1 week, and the remaining half were sacrificed after 2 weeks. Inflammation, necrosis, and new bone areas were evaluated by means of histologic and histomorphometric analyses.
Compared to the control group, defects treated with VEGF alone or in combination with BA showed higher rates of bone formation (33.10-46.60%) on Day 7. Additionally, VEGF significantly reduced inflammation and necrosis (P < 0.001). However, the differences were no longer discernable on Day 14.
VEGF makes a significant contribution to angiogenesis and osteogenesis in the early stages of bone defect healing, and its combination with an osteoconductive grafting material (BA) may further enhance new bone formation.
背景/目的：充足的骨量是口腔修复学中的一个关键因素。尽管使用了许多有前景的同种异体材料，但刺激骨形成的成功率一直有限，主要是由于局部生物学反应不佳。已引入生长因子来刺激血管生成和新骨形成。本组织学和组织形态计量学研究旨在评估血管内皮生长因子（VEGF）和双相异体移植材料（BA）对大鼠骨内缺损愈合的影响。
使用20只雄性Wistar大鼠。在每只大鼠的左右股骨上均制造两个临界尺寸的骨缺损。每个缺损随机分配接受VEGF、BA、VEGF + BA治疗，或留空作为对照。一半动物在1周后处死，另一半在2周后处死。通过组织学和组织形态计量学分析评估炎症、坏死和新骨区域。
与对照组相比，单独使用VEGF或与BA联合治疗的缺损在第7天显示出更高的骨形成率（33.10 - 46.60%）。此外，VEGF显著减少了炎症和坏死（P < 0.001）。然而，在第14天差异不再明显。
VEGF在骨缺损愈合的早期阶段对血管生成和成骨有显著贡献，并且它与骨传导性移植材料（BA）联合使用可能进一步增强新骨形成。
