Lee Hsin-Lin, Wang Ren-Syue, Hsu Yung-Chuang, Chuang Chuan-Chung, Chan Hui-Rong, Chiu Hsien-Chung, Wang Yi-Bing, Chen Kuo-Yu, Fu Earl
Department of Dentistry, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan.
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.
J Dent Sci. 2018 Jun;13(2):160-166. doi: 10.1016/j.jds.2017.06.004. Epub 2018 Feb 9.
BACKGROUND/PURPOSE: Denture stomatitis is a pathological condition affecting the mucosa underneath ill-fitting dentures, and is considered its main etiologic factor. Tissue conditioners are temporary lining materials often applied to dentures to treat inflamed tissues. However, tissue conditioners do not exert antifungal activity, and the soft surface texture harbors easily. The aim of this study was to examine the antifungal activity of tissue conditioners modified with chitosan (CS) or a quaternized chitosan (QCS), which was synthesized by grafting 2-[(acryloyloxy)ethyl] trimethyl ammonium chloride onto CS.
Tissue conditioners containing varying weight percentages of CS or QCS were prepared as experimental discs 10 mm in diameter and 1 mm in thickness. Samples were co-cultured with and the number of colony forming units was recorded. Other evaluations included cell toxicity and tensile bond strength to the resin denture base.
It was found significantly fewer fungal colonies in tissue conditioners modified with CS or QCS, and none when the weight percentage of QCS exceeded 5%. CS and QCS did not affect the viability of human gingival epithelium cells or fibroblasts, and tensile bond strength did not differ between control and modified tissue conditioners.
This study provides a foundation for the development of QCS as a novel and safe antifungal agent applied to tissue conditioners in clinical practice.
背景/目的：义齿性口炎是一种影响不合适义齿下方黏膜的病理状况，被认为是其主要病因。组织调理剂是常用于义齿以治疗炎症组织的临时衬里材料。然而，组织调理剂不具有抗真菌活性，且其柔软的表面质地容易滋生微生物。本研究的目的是检测用壳聚糖（CS）或季铵化壳聚糖（QCS）改性的组织调理剂的抗真菌活性，QCS是通过将2-[(丙烯酰氧基)乙基]三甲基氯化铵接枝到CS上合成的。
制备含有不同重量百分比CS或QCS的组织调理剂作为直径10毫米、厚度1毫米的实验圆盘。样品与[具体微生物名称未给出]共培养，并记录菌落形成单位的数量。其他评估包括细胞毒性和与树脂义齿基托的拉伸粘结强度。
发现在用CS或QCS改性的组织调理剂中真菌菌落明显减少，当QCS的重量百分比超过5%时则无真菌菌落。CS和QCS不影响人牙龈上皮细胞或成纤维细胞的活力，对照和改性组织调理剂之间的拉伸粘结强度没有差异。
本研究为开发QCS作为一种新型安全的抗真菌剂应用于临床实践中的组织调理剂奠定了基础。
