Saeed Asfia, Zahid Shahreen, Sajid Muhammad, Ud Din Shahab, Alam Mohammad Khursheed, Chaudhary Farooq Ahmad, Kaleem Muhammad, Alswairki Haytham Jamil, Abutayyem Huda
Department of Dental Materials, Army Medical College, National University of Medical Sciences, Rawalpindi 46000, Pakistan.
Department of Dental Materials, Islamabad Medical & Dental College, Islamabad 45400, Pakistan.
Polymers (Basel). 2022 Mar 18;14(6):1233. doi: 10.3390/polym14061233.
This study aims to compare the hardness, sorption and solubility of commercially available tissue conditioner [TC] modified with chitosan [CS] and synthesized chitosan oligosaccharide [COS] in antifungal concentration. COS was synthesized by acid hydrolysis and characterized by FTIR and XRD. Experimental materials were formulated by incorporating each per gram of TC powder with effective antifungal concentration of chitosan 1.02 mg (Group 1: TC-CS) and 0.51 mg COS (Group 2: TC-COS). A commercially available TC was used as control (Group 0: CTC). Shore A hardness test was performed according to ASTM D 2240-05 (2010) standards on samples stored in dry environment, distilled water (DW) and artificial saliva (AS) at 37 °C ( = 5 per group). Percent weight changes ( = 5 per group) after storage in DW and AS was used to record sorption and solubility. One-way Anova with post hoc Tukey's test was applied. FTIR and XRD confirmed low molecular weight and amorphous nature of COS. Experimental groups had higher Shore A hardness values; however, these changes were not significant. Greatest variations in durometer values ( ≤ 0.05) were observed during the first 24 h. Experimental groups had higher ( ≤ 0.05) percentage sorption and solubility. Samples stored in DW had significantly higher ( = 0.019) sorption, whereas material had higher ( = 0.005) solubility in AS. Mean solubility values in both immersion mediums was highest for Group 2, followed by group 1 and group 0. In addition, significant ( ≤ 0.05) increase in solubility upon aging was noted for each material. Experimental tissue conditioner had higher hardness, sorption and solubility. However, these changes are not substantial to interfere with their tissue healing property. Therefore, these materials may be considered and explored further as potential antimicrobial drug delivery agent for denture stomatitis patients.
本研究旨在比较市售组织调理剂[TC]用壳聚糖[CS]和合成壳寡糖[COS]在抗真菌浓度下改性后的硬度、吸附性和溶解性。COS通过酸水解合成，并通过傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）进行表征。通过将每克TC粉末与有效抗真菌浓度的1.02毫克壳聚糖（第1组：TC-CS）和0.51毫克COS（第2组：TC-COS）混合来配制实验材料。使用市售的TC作为对照（第0组：CTC）。根据ASTM D 2240-05（2010）标准，对储存在干燥环境、蒸馏水（DW）和人工唾液（AS）中于37°C的样品进行肖氏A硬度测试（每组n = 5）。将样品储存在DW和AS中后的重量变化百分比（每组n = 5）用于记录吸附性和溶解性。应用单因素方差分析及事后Tukey检验。FTIR和XRD证实了COS的低分子量和无定形性质。实验组的肖氏A硬度值较高；然而，这些变化并不显著。在前24小时内观察到硬度计值的最大变化（p≤0.05）。实验组具有更高（p≤0.05）的吸附百分比和溶解性。储存在DW中的样品具有显著更高（p = 0.019）的吸附性，而材料在AS中具有更高（p = 0.005）的溶解性。在两种浸泡介质中，第2组的平均溶解度值最高，其次是第1组和第0组。此外，每种材料在老化后溶解度均有显著（p≤0.05）增加。实验性组织调理剂具有更高的硬度、吸附性和溶解性。然而，这些变化并不足以干扰其组织愈合性能。因此，这些材料可作为义齿性口炎患者潜在的抗菌药物递送剂进一步考虑和探索。
