间充质干细胞对小鼠颅骨骨缺损破骨细胞前体细胞趋化作用的动态成像
Dynamic imaging of the effect of mesenchymal stem cells on osteoclast precursor cell chemotaxis for bone defects in the mouse skull.
作者信息
Abe Takaharu, Sumi Keisuke, Kunimatsu Ryo, Oki Nanae, Tsuka Yuji, Nakajima Kengo, Tanimoto Kotaro
机构信息
Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Japan.
Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical & Health Sciences, Japan.
出版信息
J Dent Sci. 2018 Dec;13(4):354-359. doi: 10.1016/j.jds.2018.08.001. Epub 2018 Aug 24.
BACKGROUND/PURPOSE: Mesenchymal stem cells (MSCs) transplantation has previously been used in the field of regenerative medicine. Although bone regeneration is known to occur through the interaction between osteoblasts and osteoclasts, the effect of MSCs on osteoclasts is unknown. Therefore, the purpose of this study was to investigate the effect of MSCs on the chemotaxis of osteoclast precursor cells (RAW264 macrophage cells).
MATERIALS AND METHODS
Bone defects were created in mice skulls, and MSCs and a scaffold of carbonated hydroxyapatite were transplanted into the bone defects. RAW264 cells were then transplanted into the mouse tail vein, and their dynamics were observed by an imaging system.
RESULTS
The fluorescent intensity of the MSCs transplant group at the bone defect region was significantly higher on days 3, 5, and 7 compared with the MSCs non-transplant group.
CONCLUSION
Increased RAW264 chemotaxis to the bone defect region occurred following the simultaneous implantation of MSCs in the skull defect.
背景/目的：间充质干细胞（MSCs）移植先前已用于再生医学领域。虽然已知骨再生是通过成骨细胞和破骨细胞之间的相互作用发生的，但MSCs对破骨细胞的作用尚不清楚。因此，本研究的目的是探讨MSCs对破骨细胞前体细胞（RAW264巨噬细胞）趋化性的影响。
材料与方法
在小鼠颅骨上制造骨缺损，将MSCs和碳酸羟基磷灰石支架移植到骨缺损处。然后将RAW264细胞移植到小鼠尾静脉中，并通过成像系统观察其动态变化。
结果
与未移植MSCs的组相比，移植MSCs组在骨缺损区域的荧光强度在第3、5和7天显著更高。
结论
在颅骨缺损处同时植入MSCs后，RAW264对骨缺损区域的趋化性增加。
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